Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 7303..7904 Replicon plasmid pL73-2
Accession NZ_CP033379
Organism Escherichia coli strain L73

Toxin (Protein)


Gene name doc Uniprot ID -
Locus tag EB670_RS23780 Protein ID WP_001216039.1
Coordinates 7524..7904 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID -
Locus tag EB670_RS23775 Protein ID WP_001190711.1
Coordinates 7303..7524 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB670_RS23740 2675..3372 + 698 Protein_4 IS1 family transposase -
EB670_RS23745 3388..3663 + 276 Protein_5 serine/threonine protein phosphatase -
EB670_RS23750 3660..4361 + 702 WP_000684873.1 hypothetical protein -
EB670_RS23760 4663..5925 + 1263 WP_069067570.1 hypothetical protein -
EB670_RS23765 5998..6324 + 327 Protein_8 hypothetical protein -
EB670_RS23770 6341..7038 - 698 Protein_9 IS1 family transposase -
EB670_RS23775 7303..7524 + 222 WP_001190711.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EB670_RS23780 7524..7904 + 381 WP_001216039.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EB670_RS23785 7909..8088 + 180 WP_000113018.1 hypothetical protein -
EB670_RS23790 8116..9159 + 1044 WP_069067567.1 DUF968 domain-containing protein -
EB670_RS23800 9462..9773 + 312 WP_001351993.1 phage antirepressor Ant -
EB670_RS23805 9806..10657 - 852 WP_000611661.1 hypothetical protein -
EB670_RS23810 10768..10977 - 210 WP_000874156.1 hypothetical protein -
EB670_RS23825 11582..11803 + 222 WP_000542338.1 hypothetical protein -
EB670_RS23830 11811..12842 + 1032 WP_000067537.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qnrS1 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / floR / tet(A) mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..167170 167170


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13558.27 Da        Isoelectric Point: 5.1514

>T113582 WP_001216039.1 NZ_CP033379:7524-7904 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRAALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T113582 NZ_CP033379:7524-7904 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTGCCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8134.08 Da        Isoelectric Point: 4.5843

>AT113582 WP_001190711.1 NZ_CP033379:7303-7524 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVDR

Download         Length: 222 bp

>AT113582 NZ_CP033379:7303-7524 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCTCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTGACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References