Detailed information of TA system TA113579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4143768..4144344
• Replicon: chromosome
• Accession: NZ_CP033378
• Organism: Escherichia coli strain L73

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: EB670_RS20400
• Protein ID: WP_024184631.1
• Coordinates: 4144057..4144344 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: EB670_RS20395
• Protein ID: WP_072841999.1
• Coordinates: 4143768..4144070 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB670_RS20375	4139459..4139971	+	513	WP_001175455.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-
EB670_RS20380	4140364..4142514	+	2151	WP_001309197.1	pyruvate/proton symporter BtsT	-
EB670_RS20385	4142564..4142767	+	204	WP_000467859.1	YbdD/YjiX family protein	-
EB670_RS20390	4142778..4143734	+	957	WP_001295525.1	GTPase	-
EB670_RS20395	4143768..4144070	-	303	WP_072841999.1	BrnA antitoxin family protein	Antitoxin
EB670_RS20400	4144057..4144344	-	288	WP_024184631.1	BrnT family toxin	Toxin
EB670_RS20405	4144541..4145707	+	1167	WP_000800826.1	restriction endonuclease	-
EB670_RS20410	4145771..4147390	+	1620	WP_001029745.1	type I restriction-modification system subunit M	-
EB670_RS20415	4147380..4148141	+	762	Protein_3957	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11184.68 Da
• Isoelectric Point: 7.4697

>T113579 WP_024184631.1 NZ_CP033378:c4144344-4144057 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
VRKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T113579 NZ_CP033378:c4144344-4144057 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGCATTGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GTACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11546.05 Da
• Isoelectric Point: 9.4646

>AT113579 WP_072841999.1 NZ_CP033378:c4144070-4143768 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEEIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT113579 NZ_CP033378:c4144070-4143768 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACGCCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGAGATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA