Detailed information of TA system TA113577

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3778227..3779025
• Replicon: chromosome
• Accession: NZ_CP033378
• Organism: Escherichia coli strain L73

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A166U9J9
• Locus tag: EB670_RS18645
• Protein ID: WP_000854695.1
• Coordinates: 3778227..3778604 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: EB670_RS18650
• Protein ID: WP_001351626.1
• Coordinates: 3778651..3779025 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB670_RS18615	3773505..3774461	+	957	WP_000121346.1	molybdenum cofactor insertion chaperone PaoD	-
EB670_RS18620	3774512..3774850	+	339	Protein_3611	LysR family transcriptional regulator	-
EB670_RS18625	3775188..3776507	+	1320	WP_000144689.1	site-specific integrase	-
EB670_RS18630	3776600..3777448	-	849	Protein_3613	DUF4942 domain-containing protein	-
EB670_RS18635	3777533..3777730	-	198	WP_000839228.1	DUF957 domain-containing protein	-
EB670_RS18640	3777742..3778230	-	489	WP_000761710.1	hypothetical protein	-
EB670_RS18645	3778227..3778604	-	378	WP_000854695.1	TA system toxin CbtA family protein	Toxin
EB670_RS18650	3778651..3779025	-	375	WP_001351626.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EB670_RS18655	3779188..3779409	-	222	WP_000692307.1	DUF987 domain-containing protein	-
EB670_RS18660	3779478..3779954	-	477	WP_001186773.1	RadC family protein	-
EB670_RS18665	3779970..3780449	-	480	WP_000860084.1	antirestriction protein	-
EB670_RS18670	3780531..3781349	-	819	WP_001241366.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / fdeC / fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3748675..3780449	31774

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14062.03 Da
• Isoelectric Point: 7.7531

>T113577 WP_000854695.1 NZ_CP033378:c3778604-3778227 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPGAKQ

Nucleotide

• Length: 378 bp

>T113577 NZ_CP033378:c3778604-3778227 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGCACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13838.71 Da
• Isoelectric Point: 7.0266

>AT113577 WP_001351626.1 NZ_CP033378:c3779025-3778651 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFRDADVYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT113577 NZ_CP033378:c3779025-3778651 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGAGATGCAGATGTGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A166U9J9