Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3591790..3592408 Replicon chromosome
Accession NZ_CP033378
Organism Escherichia coli strain L73

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EB670_RS17750 Protein ID WP_001291435.1
Coordinates 3592190..3592408 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EB670_RS17745 Protein ID WP_000344800.1
Coordinates 3591790..3592164 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB670_RS17735 3586879..3588072 + 1194 WP_001351640.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EB670_RS17740 3588095..3591244 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
EB670_RS17745 3591790..3592164 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EB670_RS17750 3592190..3592408 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
EB670_RS17755 3592580..3593131 + 552 WP_000102564.1 maltose O-acetyltransferase -
EB670_RS17760 3593247..3593717 + 471 WP_001351639.1 YlaC family protein -
EB670_RS17765 3593881..3595431 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EB670_RS17770 3595473..3595826 - 354 WP_000878140.1 DUF1428 domain-containing protein -
EB670_RS17780 3596205..3596516 + 312 WP_000409911.1 MGMT family protein -
EB670_RS17785 3596547..3597119 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T113576 WP_001291435.1 NZ_CP033378:3592190-3592408 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T113576 NZ_CP033378:3592190-3592408 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT113576 WP_000344800.1 NZ_CP033378:3591790-3592164 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT113576 NZ_CP033378:3591790-3592164 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References