Detailed information of TA system TA113555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 3530094..3530764
• Replicon: chromosome
• Accession: NZ_CP033367
• Organism: Mesorhizobium loti R88b

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EB235_RS17315
• Protein ID: WP_027029806.1
• Coordinates: 3530366..3530764 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6M7WWB6
• Locus tag: EB235_RS17310
• Protein ID: WP_027029807.1
• Coordinates: 3530094..3530366 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB235_RS17290	3526179..3526742	+	564	WP_032925432.1	gluconokinase	-
EB235_RS17295	3526848..3527981	-	1134	WP_027029810.1	hypothetical protein	-
EB235_RS17300	3528174..3528623	-	450	WP_027029809.1	Rrf2 family transcriptional regulator	-
EB235_RS17305	3528871..3529857	-	987	WP_027029808.1	sulfate ABC transporter substrate-binding protein	-
EB235_RS17310	3530094..3530366	+	273	WP_027029807.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EB235_RS17315	3530366..3530764	+	399	WP_027029806.1	type II toxin-antitoxin system VapC family toxin	Toxin
EB235_RS17320	3530779..3531819	-	1041	WP_027029805.1	sulfate/molybdate ABC transporter ATP-binding protein	-
EB235_RS17325	3531833..3532717	-	885	WP_032925431.1	sulfate ABC transporter permease subunit CysW	-
EB235_RS17330	3532710..3533561	-	852	WP_027029803.1	sulfate ABC transporter permease subunit CysT	-
EB235_RS17335	3533584..3534612	-	1029	WP_027029802.1	sulfate ABC transporter substrate-binding protein	-
EB235_RS17340	3534833..3535555	-	723	WP_051429598.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-116)

Antitoxin

• SpoVT-AbrB domain (17-55)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14304.76 Da
• Isoelectric Point: 5.7547

>T113555 WP_027029806.1 NZ_CP033367:3530366-3530764 [Mesorhizobium loti R88b]
MRFMLDTNIISDMIRNPAGKAAGAMVREGDAAVCTSIVVASELRYGCARKGSAKLLRKVEELLAEIPVLPLDVPVDAEYG
ALRAELEAIGQPIGHNDLFIAAHACVLGTILVTANMGEFTRIRKLKVENWLE

Nucleotide

• Length: 399 bp

>T113555 NZ_CP033367:3530366-3530764 [Mesorhizobium loti R88b]
ATGCGGTTCATGCTGGACACGAACATCATCAGCGACATGATCCGAAACCCGGCCGGCAAGGCTGCCGGCGCTATGGTGCG
CGAGGGAGACGCAGCCGTCTGCACAAGCATCGTCGTCGCCTCTGAGTTGCGATATGGCTGCGCCAGGAAAGGCTCGGCCA
AGCTGCTCAGGAAGGTCGAGGAATTGCTGGCCGAAATCCCTGTTCTGCCGCTCGATGTGCCGGTGGATGCAGAGTATGGC
GCACTTCGCGCCGAGCTGGAGGCCATTGGCCAACCCATTGGCCATAATGACCTTTTTATTGCCGCTCACGCCTGCGTGCT
GGGTACCATTTTGGTGACGGCCAACATGGGGGAGTTCACCCGGATACGGAAGCTGAAGGTCGAGAACTGGCTGGAATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10147.55 Da
• Isoelectric Point: 5.1473

>AT113555 WP_027029807.1 NZ_CP033367:3530094-3530366 [Mesorhizobium loti R88b]
MSQHRHPTTPPKEAKLFRNNRSQAVRIPVEFELPGDKVLISREGDRLVIEPVRKPGLSALLAQWAKEPPLGPDDEFPEIN
DAPVQAEDIF

Nucleotide

• Length: 273 bp

>AT113555 NZ_CP033367:3530094-3530366 [Mesorhizobium loti R88b]
ATGTCCCAGCACCGTCATCCAACGACGCCACCGAAGGAGGCAAAGCTCTTCCGCAACAACCGCAGCCAGGCGGTGCGCAT
TCCTGTGGAGTTCGAGCTGCCGGGTGACAAGGTGCTGATCAGCCGCGAAGGTGATCGCCTTGTCATCGAGCCGGTTCGCA
AACCTGGCCTCAGCGCGTTGCTTGCCCAATGGGCGAAGGAGCCACCGCTCGGTCCTGACGACGAATTCCCCGAGATCAAC
GATGCGCCGGTCCAGGCCGAGGACATTTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A6M7WWB6