Detailed information of TA system TA113554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3380508..3382138
• Replicon: chromosome
• Accession: NZ_CP033367
• Organism: Mesorhizobium loti R88b

Toxin (Protein)

• Gene name: HipA2
• Uniprot ID: A0A6M7WVB2
• Locus tag: EB235_RS16490
• Protein ID: WP_027029953.1
• Coordinates: 3380786..3382138 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: HipB2
• Uniprot ID: -
• Locus tag: EB235_RS34590
• Protein ID: WP_208603653.1
• Coordinates: 3380508..3380786 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB235_RS16460	3375886..3377055	+	1170	WP_171878176.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
EB235_RS16465	3377079..3377357	-	279	WP_027029958.1	hypothetical protein	-
EB235_RS16470	3377765..3378280	+	516	WP_027029957.1	hypothetical protein	-
EB235_RS16475	3378381..3378998	+	618	WP_027029956.1	C39 family peptidase	-
EB235_RS16480	3379014..3380087	+	1074	WP_027029955.1	D-alanine--D-alanine ligase	-
EB235_RS34590	3380508..3380786	+	279	WP_208603653.1	type II toxin-antitoxin system Y4mF family antitoxin	Antitoxin
EB235_RS16490	3380786..3382138	+	1353	WP_027029953.1	HipA domain-containing protein	Toxin
EB235_RS16495	3382177..3383124	-	948	WP_027029952.1	SDR family oxidoreductase	-
EB235_RS16500	3383121..3383990	-	870	WP_027029951.1	SDR family NAD(P)-dependent oxidoreductase	-
EB235_RS16505	3384108..3384677	-	570	WP_027029950.1	TetR/AcrR family transcriptional regulator	-
EB235_RS16510	3384875..3386824	+	1950	WP_027029949.1	AI-2E family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (156-385)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-87)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 48395.80 Da
• Isoelectric Point: 5.5050

>T113554 WP_027029953.1 NZ_CP033367:3380786-3382138 [Mesorhizobium loti R88b]
MDALSLDVRLDGFVEPIGVLARDSNGAVAYAYRSDYVTSPDAVALSLSLPLTDEPYGDVAARPFFDNLLQERDAALADIM
AREGLARDDIAGLLFHLGKDCAGALSVLPAGAPSVKLPGDYGRDYIPVGGERMEQIVDALHRRRRLPDGTADPSPLAGVQ
SKIALTMLPDGSFGEPRPGSGAPTTHILKVPDQGHLHDARDEAEALSLSRALGFETTDAVVVPFDAVGALLITRFDRART
RDGRIVRIHQEDFAQALGFPAALKYERRGTPGRRFDAPAIRRVLEATADPSDAKEIFVRATLFDLMIGNTDGHAKNFALY
HEARGRIRMAPRYDLLPTRLDANLTDELAYRIGSADRLEAISADDFSAFLQALGIDSAAARKRMRAGYTADIGGSLAQQL
AGLDKRNMKRFADLIASNIATLTAAFGVGTPAAVGQRDAFLDRAGGWLMS

Nucleotide

• Length: 1353 bp

>T113554 NZ_CP033367:3380786-3382138 [Mesorhizobium loti R88b]
ATGGACGCGCTGTCGCTCGATGTCCGGCTCGACGGGTTCGTCGAGCCGATCGGCGTCCTGGCGCGGGACAGCAATGGCGC
GGTCGCCTATGCCTACCGGTCGGACTATGTCACCAGTCCCGATGCAGTCGCCTTGTCACTGTCGCTGCCGTTGACCGACG
AGCCCTATGGCGACGTTGCGGCCCGGCCGTTCTTCGACAATCTGCTGCAGGAGCGTGACGCGGCACTGGCCGACATCATG
GCCCGCGAGGGTCTCGCCCGCGACGACATTGCCGGTCTGTTGTTCCATCTCGGCAAGGACTGCGCCGGCGCCCTGTCTGT
GCTGCCTGCGGGTGCGCCTTCAGTCAAGCTGCCCGGTGATTATGGCCGTGACTACATACCGGTCGGAGGGGAGCGGATGG
AGCAGATCGTCGATGCGCTGCATCGCCGTCGTCGCCTGCCGGATGGCACTGCCGATCCGTCTCCGCTGGCCGGGGTGCAA
AGCAAGATCGCGCTGACCATGCTGCCCGACGGCTCCTTTGGCGAACCACGACCAGGTTCGGGCGCGCCGACCACGCACAT
ATTGAAGGTTCCCGATCAGGGGCATCTGCACGATGCCCGCGATGAGGCCGAGGCGCTCTCGCTGTCGCGAGCGCTTGGCT
TCGAGACGACTGACGCCGTGGTCGTGCCGTTCGACGCGGTCGGAGCTCTTCTGATCACCCGCTTCGACCGCGCGCGTACC
CGCGACGGCCGGATCGTGCGTATCCATCAGGAGGACTTCGCCCAGGCACTCGGCTTCCCGGCGGCGCTGAAATACGAACG
GCGTGGTACGCCCGGCCGGCGCTTCGACGCGCCGGCCATTCGCCGTGTGCTGGAGGCGACAGCGGATCCGTCGGATGCCA
AGGAGATATTCGTCAGAGCCACCCTGTTCGACCTGATGATCGGCAATACCGATGGCCACGCCAAGAATTTCGCGCTGTAT
CACGAGGCACGAGGGCGCATCCGAATGGCACCACGCTATGACCTCCTGCCGACGCGACTCGACGCCAACCTGACCGATGA
GCTTGCCTACAGGATCGGTTCGGCGGACCGACTGGAAGCAATTTCCGCCGACGACTTTTCAGCCTTCCTGCAGGCGCTCG
GCATCGATAGTGCCGCCGCGCGCAAGCGTATGCGGGCGGGCTATACGGCCGATATAGGAGGGTCGTTGGCGCAGCAGCTC
GCCGGCCTCGACAAGCGAAACATGAAGCGCTTCGCCGATCTCATCGCATCGAACATCGCGACGCTGACCGCGGCGTTTGG
GGTTGGCACACCGGCGGCGGTCGGGCAGCGCGACGCGTTTCTCGACCGGGCCGGGGGCTGGCTGATGAGCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9843.29 Da
• Isoelectric Point: 10.0842

>AT113554 WP_208603653.1 NZ_CP033367:3380508-3380786 [Mesorhizobium loti R88b]
MLESDAKEQATTPIRTKGAEAELTTIGSPADLGRLVRKVREGRGLSQQEFADLAGVGRRFLSELENGKPTLELGKVLKVA
NAAGIALLARER

Nucleotide

• Length: 279 bp

>AT113554 NZ_CP033367:3380508-3380786 [Mesorhizobium loti R88b]
ATGCTCGAATCCGATGCAAAGGAGCAGGCAACGACGCCAATCAGAACCAAGGGGGCCGAGGCCGAACTCACCACCATCGG
ATCGCCGGCCGATCTCGGCCGGCTGGTGCGCAAGGTGCGTGAGGGGCGGGGGCTTTCGCAACAGGAATTCGCCGATCTCG
CCGGCGTCGGCAGGCGTTTCCTGTCCGAGCTTGAGAACGGCAAGCCGACGCTGGAATTGGGCAAGGTGCTGAAGGTCGCC
AATGCCGCCGGCATCGCGCTTCTCGCCAGGGAACGTTGA

Structures

Toxin

• AlphaFold DB: A0A6M7WVB2