Detailed information of TA system TA113553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relE/YoeB-YefM
• Location: 3022445..3022950
• Replicon: chromosome
• Accession: NZ_CP033367
• Organism: Mesorhizobium loti R88b

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A6M7WM15
• Locus tag: EB235_RS14690
• Protein ID: WP_027030273.1
• Coordinates: 3022445..3022699 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EB235_RS14695
• Protein ID: WP_027030272.1
• Coordinates: 3022696..3022950 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB235_RS14655	3017487..3017954	+	468	WP_027030280.1	GNAT family N-acetyltransferase	-
EB235_RS14660	3017982..3018365	+	384	WP_027030279.1	ACT domain-containing protein	-
EB235_RS14665	3018517..3019950	+	1434	WP_027030278.1	aldehyde dehydrogenase family protein	-
EB235_RS14670	3019966..3020454	-	489	WP_208603625.1	translation initiation factor 2	-
EB235_RS14675	3020612..3020830	+	219	WP_155256387.1	hypothetical protein	-
EB235_RS14680	3021257..3021667	+	411	WP_027030275.1	MbcA/ParS/Xre antitoxin family protein	-
EB235_RS14685	3021661..3022359	+	699	WP_027030274.1	RES family NAD+ phosphorylase	-
EB235_RS14690	3022445..3022699	-	255	WP_027030273.1	Txe/YoeB family addiction module toxin	Toxin
EB235_RS14695	3022696..3022950	-	255	WP_027030272.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
EB235_RS14700	3023139..3023435	+	297	WP_202596386.1	helix-turn-helix domain-containing protein	-
EB235_RS14705	3023478..3024593	+	1116	WP_027030270.1	alkene reductase	-
EB235_RS14710	3024785..3025549	+	765	WP_027030269.1	substrate-binding domain-containing protein	-
EB235_RS14715	3025804..3026853	+	1050	WP_027030268.1	ABC transporter substrate-binding protein	-
EB235_RS14720	3026857..3027894	+	1038	WP_027030267.1	iron ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9956.40 Da
• Isoelectric Point: 9.5797

>T113553 WP_027030273.1 NZ_CP033367:c3022699-3022445 [Mesorhizobium loti R88b]
VKLIFHERAWEDYLHWQATDAKLLARVNALIKECTRTPFTGTGKPEPLRGPLSGWWSRRITQEHRLVYRAGEDSLFIAQC
RYHY

Nucleotide

• Length: 255 bp

>T113553 NZ_CP033367:c3022699-3022445 [Mesorhizobium loti R88b]
GTGAAGCTGATCTTCCATGAACGCGCATGGGAGGATTATCTGCACTGGCAGGCAACCGACGCCAAACTGCTTGCACGCGT
AAATGCGCTCATCAAGGAATGCACCCGCACGCCGTTTACCGGGACAGGCAAGCCAGAACCGTTGCGTGGGCCACTTTCGG
GATGGTGGTCACGGCGCATCACGCAGGAGCATCGTCTGGTCTACCGCGCAGGCGAAGACAGCCTGTTCATCGCGCAATGT
CGCTACCACTATTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9480.48 Da
• Isoelectric Point: 4.5098

>AT113553 WP_027030272.1 NZ_CP033367:c3022950-3022696 [Mesorhizobium loti R88b]
MRTTSYSDLRRNLAATIDSVNADHEPVIITRDRGKPAAVLMSVEDFASYEETRYLLRSPRNADRLLEATQGLDQGEGTER
ELPE

Nucleotide

• Length: 255 bp

>AT113553 NZ_CP033367:c3022950-3022696 [Mesorhizobium loti R88b]
ATGCGAACCACATCCTACAGTGACCTCAGGCGCAACCTGGCCGCTACGATAGACAGCGTGAACGCTGATCATGAGCCGGT
CATCATCACGCGCGACCGCGGCAAGCCGGCGGCAGTGCTGATGTCGGTGGAGGATTTCGCCTCCTACGAGGAAACCCGCT
ATCTCCTGCGCAGCCCGCGTAACGCCGATCGCTTGCTTGAGGCGACGCAGGGATTGGACCAAGGCGAAGGGACCGAGCGC
GAGCTTCCCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A6M7WM15