Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2823642..2824291 Replicon chromosome
Accession NZ_CP033367
Organism Mesorhizobium loti R88b

Toxin (Protein)


Gene name vapC Uniprot ID A0A6M7WLG6
Locus tag EB235_RS13705 Protein ID WP_027030450.1
Coordinates 2823642..2824031 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6M7WK53
Locus tag EB235_RS13710 Protein ID WP_027030449.1
Coordinates 2824028..2824291 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB235_RS13680 2819346..2819972 - 627 WP_027030455.1 TetR/AcrR family transcriptional regulator -
EB235_RS13685 2820039..2820464 + 426 WP_027030454.1 VOC family protein -
EB235_RS13690 2820869..2821816 - 948 WP_027030453.1 trypsin-like peptidase domain-containing protein -
EB235_RS13695 2822044..2822916 + 873 WP_027030452.1 S1C family serine protease -
EB235_RS13700 2822925..2823635 + 711 WP_155256402.1 helix-turn-helix transcriptional regulator -
EB235_RS13705 2823642..2824031 - 390 WP_027030450.1 type II toxin-antitoxin system VapC family toxin Toxin
EB235_RS13710 2824028..2824291 - 264 WP_027030449.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
EB235_RS13715 2824468..2825334 + 867 WP_027030448.1 NAD(P)-dependent oxidoreductase -
EB235_RS13720 2825354..2825869 - 516 WP_032925503.1 TIGR00645 family protein -
EB235_RS13725 2826290..2828614 - 2325 WP_027030447.1 OmpA family protein -
EB235_RS13730 2828813..2829259 + 447 WP_027030446.1 YcgN family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-123)

Antitoxin

Antitoxin VapB-like

(1-85)


Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14240.38 Da        Isoelectric Point: 4.9146

>T113552 WP_027030450.1 NZ_CP033367:c2824031-2823642 [Mesorhizobium loti R88b]
MIIDSSAIVAILRSEADRDIYLSAIDRAEIRRIPAPNLVETTMVLTGLHRSDMMEELDAFLRTASIETIAFTADHAAVAR
QAFLRYGKGRHPAALNFGDCIAYATARLEAMPLLFKGDDFRLTDIEAAV

Download         Length: 390 bp

>T113552 NZ_CP033367:c2824031-2823642 [Mesorhizobium loti R88b]
ATGATCATTGATTCATCGGCAATCGTTGCGATCCTGCGATCCGAGGCCGATCGCGACATCTACCTCTCCGCCATAGATCG
TGCCGAAATACGCAGAATTCCGGCACCCAACCTCGTTGAAACAACGATGGTCCTCACGGGACTGCATCGATCCGATATGA
TGGAAGAATTGGATGCGTTTCTACGCACTGCGTCGATAGAGACAATCGCCTTCACCGCCGACCACGCAGCTGTCGCCCGC
CAGGCGTTTCTGCGTTACGGCAAGGGCCGACATCCGGCGGCGCTGAACTTCGGCGACTGTATCGCCTACGCCACGGCAAG
GCTCGAAGCCATGCCGCTGCTGTTCAAGGGTGATGACTTCCGACTGACCGACATCGAAGCGGCGGTGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10085.50 Da        Isoelectric Point: 6.4136

>AT113552 WP_027030449.1 NZ_CP033367:c2824291-2824028 [Mesorhizobium loti R88b]
MALSIKNTEVEELARELARRRRVSVTEAIRQSLEREVARERLVPRPKDSDLLTKLREISDRAGRIPRISDMTDDEILGYD
ELGIPNR

Download         Length: 264 bp

>AT113552 NZ_CP033367:c2824291-2824028 [Mesorhizobium loti R88b]
ATGGCACTCTCGATCAAGAACACGGAAGTCGAAGAACTGGCGCGAGAACTCGCCCGGCGCCGCCGCGTGTCCGTGACGGA
GGCGATCCGCCAGAGCCTCGAGCGCGAGGTCGCGCGCGAACGGCTGGTGCCGAGACCAAAAGACTCTGATTTACTAACAA
AGCTTAGGGAAATTTCTGACCGAGCGGGTCGCATCCCACGCATCTCCGACATGACGGATGATGAGATTCTCGGATACGAT
GAGCTGGGCATTCCGAACCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6M7WLG6

Antitoxin

Source ID Structure
AlphaFold DB A0A6M7WK53

References