Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 1149754..1150331 Replicon chromosome
Accession NZ_CP033367
Organism Mesorhizobium loti R88b

Toxin (Protein)


Gene name PumA Uniprot ID A0A6M7WJY7
Locus tag EB235_RS05505 Protein ID WP_027031962.1
Coordinates 1150044..1150331 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A6M7WZ37
Locus tag EB235_RS05500 Protein ID WP_027031963.1
Coordinates 1149754..1150041 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB235_RS05470 1144870..1145268 - 399 WP_027031969.1 hypothetical protein -
EB235_RS05475 1145368..1145571 - 204 WP_027031968.1 hypothetical protein -
EB235_RS05480 1145973..1146722 + 750 WP_027031967.1 hypothetical protein -
EB235_RS05485 1147026..1147427 - 402 WP_027031966.1 30S ribosomal protein S16 -
EB235_RS05490 1147477..1147803 - 327 WP_027031965.1 chorismate mutase -
EB235_RS05495 1147811..1149406 - 1596 WP_027031964.1 signal recognition particle protein -
EB235_RS05500 1149754..1150041 - 288 WP_027031963.1 putative addiction module antidote protein Antitoxin
EB235_RS05505 1150044..1150331 - 288 WP_027031962.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EB235_RS05510 1150422..1151366 - 945 WP_027031961.1 CPBP family intramembrane metalloprotease -
EB235_RS05515 1151425..1152483 - 1059 WP_027031960.1 putative zinc-binding metallopeptidase -
EB235_RS05520 1152848..1153645 + 798 WP_027031959.1 lytic transglycosylase family protein -
EB235_RS05525 1153646..1154194 + 549 WP_155256436.1 hypothetical protein -
EB235_RS05530 1154295..1154738 - 444 WP_027031957.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-94)

Antitoxin

Uncharacterized protein HI1420

(7-91)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10728.51 Da        Isoelectric Point: 10.2926

>T113551 WP_027031962.1 NZ_CP033367:c1150331-1150044 [Mesorhizobium loti R88b]
VIEVRQTTAFRDWLNSLKDKRAVEKIAIRIARIQSGLTGDVKYFDGIGELRLDFGPGYRLYFVKRGNEVIILLCGGDKSG
QGRDIKKAIQMAKEL

Download         Length: 288 bp

>T113551 NZ_CP033367:c1150331-1150044 [Mesorhizobium loti R88b]
GTGATCGAGGTTCGCCAAACAACAGCTTTTCGCGACTGGCTCAACAGTCTGAAAGACAAGCGGGCCGTCGAAAAGATCGC
GATCCGCATTGCCCGTATCCAGTCCGGCCTCACGGGCGACGTGAAGTACTTCGATGGTATCGGCGAACTTCGGTTGGATT
TTGGGCCCGGATACCGGCTCTATTTCGTCAAGCGCGGCAATGAAGTCATCATCCTGCTTTGCGGCGGCGACAAGTCCGGC
CAGGGTCGCGACATCAAGAAGGCCATCCAGATGGCCAAGGAGCTATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 9663.04 Da        Isoelectric Point: 5.0190

>AT113551 WP_027031963.1 NZ_CP033367:c1150041-1149754 [Mesorhizobium loti R88b]
MAIGTVPFDPAEHLGSAEGQAELLADAFATGDPSYIANALGIVARARGMTAVAKEAGVTREALYKALRPDGDPRLSTLLG
VTKALGVKITAEPAS

Download         Length: 288 bp

>AT113551 NZ_CP033367:c1150041-1149754 [Mesorhizobium loti R88b]
ATGGCAATCGGAACTGTTCCCTTTGATCCCGCCGAACATCTTGGCAGCGCCGAGGGGCAGGCCGAGCTGCTCGCTGACGC
CTTTGCGACCGGCGACCCCAGCTATATCGCGAATGCGCTTGGCATCGTTGCCCGGGCGCGCGGCATGACCGCGGTTGCAA
AGGAAGCAGGCGTCACGCGTGAAGCTCTCTACAAGGCACTGAGGCCCGACGGCGACCCACGCCTTTCGACACTGCTGGGT
GTCACCAAGGCGCTCGGAGTGAAGATCACAGCCGAGCCGGCCAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6M7WJY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6M7WZ37

References