Detailed information of TA system TA113549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2987444..2988117
• Replicon: chromosome
• Accession: NZ_CP033366
• Organism: Mesorhizobium japonicum R7A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EB234_RS14755
• Protein ID: WP_032930876.1
• Coordinates: 2987719..2988117 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6I4AZ99
• Locus tag: EB234_RS14750
• Protein ID: WP_032930877.1
• Coordinates: 2987444..2987719 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB234_RS14725	2982824..2983804	+	981	WP_032930881.1	ABC transporter permease	-
EB234_RS14730	2983801..2984787	+	987	WP_032930880.1	ABC transporter permease	-
EB234_RS14735	2984903..2985451	+	549	WP_032930879.1	gluconokinase	-
EB234_RS14740	2985535..2985984	-	450	WP_010910348.1	Rrf2 family transcriptional regulator	-
EB234_RS14745	2986233..2987219	-	987	WP_032930878.1	sulfate ABC transporter substrate-binding protein	-
EB234_RS14750	2987444..2987719	+	276	WP_032930877.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EB234_RS14755	2987719..2988117	+	399	WP_032930876.1	type II toxin-antitoxin system VapC family toxin	Toxin
EB234_RS14760	2988133..2989173	-	1041	WP_010910344.1	sulfate/molybdate ABC transporter ATP-binding protein	-
EB234_RS14765	2989186..2990070	-	885	WP_032930875.1	sulfate ABC transporter permease subunit CysW	-
EB234_RS14770	2990063..2990914	-	852	WP_010910342.1	sulfate ABC transporter permease subunit CysT	-
EB234_RS14775	2990996..2992024	-	1029	WP_032930874.1	sulfate ABC transporter substrate-binding protein	-
EB234_RS14780	2992229..2992960	-	732	WP_080680275.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

• SpoVT-AbrB domain (18-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14248.63 Da
• Isoelectric Point: 5.7489

>T113549 WP_032930876.1 NZ_CP033366:2987719-2988117 [Mesorhizobium japonicum R7A]
MRFMLDTNIISDMIRNPAGKAAGAMVREGDAAVCTSIVVASELRYGCARKGSTKLLKKVEDLLAEIPVLPLDVPVDAEYG
ALRAELEAVGQPIGHNDLFIAAHACVLGTTLVTANIGEFTRIRKLKVENWLE

Nucleotide

• Length: 399 bp

>T113549 NZ_CP033366:2987719-2988117 [Mesorhizobium japonicum R7A]
ATGCGGTTCATGCTGGATACCAACATCATCAGCGACATGATCCGGAACCCGGCCGGCAAGGCAGCCGGTGCAATGGTGCG
CGAGGGAGACGCCGCTGTGTGCACCAGCATCGTCGTTGCCTCTGAATTGCGATATGGCTGCGCCAGGAAGGGATCGACCA
AGCTGCTCAAGAAGGTCGAGGACCTGCTGGCCGAGATTCCCGTCCTGCCGCTCGACGTGCCGGTGGATGCCGAGTACGGC
GCACTTCGCGCCGAGCTGGAGGCTGTTGGCCAACCCATTGGCCACAATGACCTTTTTATTGCTGCTCACGCCTGCGTATT
GGGCACCACGCTGGTGACGGCCAACATCGGGGAGTTCACCCGGATACGGAAGCTGAAGGTCGAGAACTGGCTGGAATAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.65 Da
• Isoelectric Point: 4.8605

>AT113549 WP_032930877.1 NZ_CP033366:2987444-2987719 [Mesorhizobium japonicum R7A]
MPQHRQTTTPPPKEAKLFRNNRSQAVRIPVEFELPGDKVLISREGDRLVIEPLRKPGLAALLAQWAKEAPLGPEDSFPEI
NDAPVEAEDIF

Nucleotide

• Length: 276 bp

>AT113549 NZ_CP033366:2987444-2987719 [Mesorhizobium japonicum R7A]
ATGCCCCAGCATCGCCAGACGACAACGCCACCACCGAAGGAGGCGAAACTGTTCCGCAACAACCGCAGCCAGGCGGTGCG
CATTCCTGTCGAGTTCGAACTCCCCGGCGACAAGGTTCTGATCAGCCGCGAAGGCGACCGCCTTGTCATTGAGCCGCTCC
GCAAACCTGGGCTTGCCGCGCTGCTCGCCCAATGGGCGAAAGAGGCGCCGCTTGGTCCTGAGGACAGCTTTCCAGAGATC
AATGATGCGCCTGTCGAAGCCGAGGACATTTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A6I4AZ99