Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB-HicA
Location 2643827..2644422 Replicon chromosome
Accession NZ_CP033366
Organism Mesorhizobium japonicum R7A

Toxin (Protein)


Gene name hicA Uniprot ID A0A4Q1UJ83
Locus tag EB234_RS12980 Protein ID WP_065141637.1
Coordinates 2644234..2644422 (-) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q98J17
Locus tag EB234_RS12975 Protein ID WP_010910701.1
Coordinates 2643827..2644228 (-) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB234_RS12950 2639122..2640417 - 1296 WP_032931139.1 TIGR01244 family phosphatase -
EB234_RS12955 2640793..2641245 + 453 WP_032931138.1 SRPBCC domain-containing protein -
EB234_RS12960 2641274..2642104 - 831 WP_032931136.1 helix-turn-helix transcriptional regulator -
EB234_RS12965 2642195..2643079 + 885 WP_032931134.1 SDR family oxidoreductase -
EB234_RS12970 2643092..2643790 - 699 WP_032931133.1 RibD family protein -
EB234_RS12975 2643827..2644228 - 402 WP_010910701.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EB234_RS12980 2644234..2644422 - 189 WP_065141637.1 type II toxin-antitoxin system HicA family toxin Toxin
EB234_RS12985 2644783..2645730 + 948 WP_032931132.1 substrate-binding domain-containing protein -
EB234_RS12990 2645973..2647571 + 1599 WP_032931131.1 sugar ABC transporter ATP-binding protein -
EB234_RS12995 2647568..2648584 + 1017 WP_010910697.1 ABC transporter permease -
EB234_RS13000 2648619..2649125 - 507 WP_080680478.1 histidine phosphatase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-124)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7148.23 Da        Isoelectric Point: 11.6118

>T113548 WP_065141637.1 NZ_CP033366:c2644422-2644234 [Mesorhizobium japonicum R7A]
MNSTEIIRQLQADGWFEVARKGSHAQFKHRDKQGRVTVPHPKRDIPIGTLRSIEKQSGLKLR

Download         Length: 189 bp

>T113548 NZ_CP033366:c2644422-2644234 [Mesorhizobium japonicum R7A]
ATGAATAGCACCGAAATTATCCGCCAGTTGCAAGCTGATGGTTGGTTCGAGGTTGCCCGAAAGGGCAGCCATGCGCAATT
CAAACACCGTGACAAGCAAGGGCGGGTCACCGTCCCTCATCCCAAGCGCGATATCCCAATAGGCACTCTACGCAGCATCG
AAAAGCAATCTGGACTGAAACTGAGGTGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14185.00 Da        Isoelectric Point: 4.3919

>AT113548 WP_010910701.1 NZ_CP033366:c2644228-2643827 [Mesorhizobium japonicum R7A]
MKQYIGLIHKDAGSDFGVSFPDFPGVITAGKDLDDARSMAEEALTLHIEGLVEDGEAIPEPSSLQAVMADADNRDGVAIL
VAAKTEARKAVRINVTLPDDVLRRIDAFAEAHGYTRSGFLAKAAEKAMELESA

Download         Length: 402 bp

>AT113548 NZ_CP033366:c2644228-2643827 [Mesorhizobium japonicum R7A]
ATGAAGCAGTATATCGGACTTATCCATAAGGATGCCGGCAGCGACTTCGGTGTGTCTTTCCCTGATTTCCCAGGTGTGAT
TACCGCCGGAAAGGATCTGGATGACGCACGGTCCATGGCTGAAGAGGCGCTCACCCTCCACATCGAAGGGCTGGTCGAGG
ATGGCGAAGCCATTCCGGAGCCTTCCAGTCTGCAGGCCGTGATGGCGGACGCCGATAATCGTGACGGTGTCGCAATCCTG
GTCGCGGCCAAGACCGAGGCCAGGAAGGCGGTGCGTATCAACGTGACGTTGCCAGACGACGTTCTGCGGCGAATCGATGC
CTTTGCCGAAGCCCACGGCTATACGCGATCCGGGTTTCTCGCAAAGGCGGCTGAGAAAGCGATGGAGCTTGAATCGGCCT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q1UJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A4Q1UK54

References