Detailed information of TA system TA113544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 549193..549875
• Replicon: chromosome
• Accession: NZ_CP033364
• Organism: Mesorhizobium sp. NZP2234

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EB230_RS02515
• Protein ID: WP_172371218.1
• Coordinates: 549453..549875 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0Q8KUW5
• Locus tag: EB230_RS02510
• Protein ID: WP_056570294.1
• Coordinates: 549193..549456 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB230_RS02485	544275..545216	+	942	WP_056568850.1	sugar ABC transporter substrate-binding protein	-
EB230_RS02490	545326..546402	+	1077	WP_056568846.1	ABC transporter permease	-
EB230_RS02495	546405..547172	+	768	WP_027040720.1	sugar ABC transporter ATP-binding protein	-
EB230_RS02500	547292..548287	+	996	WP_172371217.1	inositol 2-dehydrogenase	-
EB230_RS02505	548314..549108	+	795	WP_056568841.1	SDR family oxidoreductase	-
EB230_RS02510	549193..549456	+	264	WP_056570294.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EB230_RS02515	549453..549875	+	423	WP_172371218.1	PIN domain-containing protein	Toxin
EB230_RS02520	550325..551416	+	1092	WP_172371219.1	3-deoxy-7-phosphoheptulonate synthase	-
EB230_RS02525	551464..552099	-	636	WP_172371220.1	LysE family transporter	-
EB230_RS02530	552229..552924	+	696	WP_172371221.1	HTH domain-containing protein	-
EB230_RS02535	552937..554046	-	1110	WP_056568828.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-70)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15533.85 Da
• Isoelectric Point: 4.8013

>T113544 WP_172371218.1 NZ_CP033364:549453-549875 [Mesorhizobium sp. NZP2234]
VRLLLDTNVLSEVTRPGPDARVLDWLDGLDEDRSFISVVSIAEIRGGVALMDEGRKRKALAEWLARDLPQRFEQRILPVD
EPVALAWGDLIGLAKRRGRGLSSMDGLIAATAIAQRLTLVTRNTRDFEGFGIELLDPWTA

Nucleotide

• Length: 423 bp

>T113544 NZ_CP033364:549453-549875 [Mesorhizobium sp. NZP2234]
GTGAGGCTGCTGCTCGATACGAATGTCCTGTCCGAGGTGACAAGGCCGGGGCCAGACGCGCGCGTTCTGGACTGGCTGGA
CGGGCTTGATGAGGATCGCTCATTCATCAGTGTCGTGTCGATCGCCGAGATCCGGGGTGGCGTCGCCCTCATGGACGAGG
GCCGGAAACGCAAGGCACTGGCCGAATGGCTCGCCCGAGACCTGCCGCAGCGTTTCGAGCAAAGGATTTTGCCTGTGGAC
GAGCCGGTTGCCCTGGCCTGGGGCGACCTTATAGGCCTTGCGAAGCGTCGCGGTCGCGGCCTGTCGTCAATGGACGGGCT
GATCGCAGCCACGGCGATTGCGCAGCGGCTAACCTTGGTAACGCGCAATACCAGGGATTTCGAAGGCTTTGGGATAGAAC
TCCTCGATCCCTGGACGGCATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9640.00 Da
• Isoelectric Point: 10.0862

>AT113544 WP_056570294.1 NZ_CP033364:549193-549456 [Mesorhizobium sp. NZP2234]
MLPVAKDTSWTVAGAKARLSEVIERAQSSPQTITKNGKPSVVVVSAEEWQRKTARKGTLAQFLLESPLRGADLDLERQRD
EPRDLPL

Nucleotide

• Length: 264 bp

>AT113544 NZ_CP033364:549193-549456 [Mesorhizobium sp. NZP2234]
ATGCTGCCTGTGGCCAAAGATACGAGCTGGACGGTCGCTGGGGCAAAGGCTCGGCTCTCGGAGGTTATCGAGCGCGCGCA
GTCTTCTCCGCAGACGATCACCAAAAACGGCAAACCAAGCGTGGTTGTCGTCTCGGCCGAGGAATGGCAGCGAAAGACCG
CGCGCAAGGGTACGCTTGCGCAGTTCCTGCTGGAGTCACCGCTGCGCGGCGCCGATCTCGACCTCGAGCGCCAGCGTGAC
GAACCGCGTGACCTGCCGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0Q8KUW5