Detailed information of TA system TA113542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/DUF1778(antitoxin)
• Location: 3263877..3264643
• Replicon: chromosome
• Accession: NZ_CP033362
• Organism: Mesorhizobium sp. NZP2077

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: EB232_RS16225
• Protein ID: WP_172234742.1
• Coordinates: 3264158..3264643 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: EB232_RS16220
• Protein ID: WP_172227191.1
• Coordinates: 3263877..3264158 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB232_RS16195	3259104..3260090	+	987	WP_172234740.1	ABC transporter permease	-
EB232_RS16200	3260093..3260641	+	549	WP_172227185.1	gluconokinase	-
EB232_RS16205	3260636..3261772	-	1137	WP_172227187.1	hypothetical protein	-
EB232_RS16210	3261950..3262399	-	450	WP_172234741.1	Rrf2 family transcriptional regulator	-
EB232_RS16215	3262649..3263635	-	987	WP_172227189.1	sulfate ABC transporter substrate-binding protein	-
EB232_RS16220	3263877..3264158	+	282	WP_172227191.1	DUF1778 domain-containing protein	Antitoxin
EB232_RS16225	3264158..3264643	+	486	WP_172234742.1	GNAT family N-acetyltransferase	Toxin
EB232_RS16230	3264697..3265737	-	1041	WP_172227193.1	sulfate/molybdate ABC transporter ATP-binding protein	-
EB232_RS16235	3265751..3266635	-	885	WP_172227196.1	sulfate ABC transporter permease subunit CysW	-
EB232_RS16240	3266628..3267479	-	852	WP_172227198.1	sulfate ABC transporter permease subunit CysT	-
EB232_RS16245	3267502..3268530	-	1029	WP_172227200.1	sulfate ABC transporter substrate-binding protein	-
EB232_RS16250	3268821..3269552	-	732	WP_172227202.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-76)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17035.62 Da
• Isoelectric Point: 10.0226

>T113542 WP_172234742.1 NZ_CP033362:3264158-3264643 [Mesorhizobium sp. NZP2077]
MLSAPTPLAENHDLELFQSGTESLDQWLRRRARANQVGGASRTYVVAEGARVVGYYCLSSGGLDLTSAPGPIRRNIPDPI
PIVVLGRLAVDAGWQGKGLGAALLQDAVLRAGHAATILGIRGIFVHAVSDEAKAFYQRYGFAASPKNPMTLVLSLKGLVK
G

Nucleotide

• Length: 486 bp

>T113542 NZ_CP033362:3264158-3264643 [Mesorhizobium sp. NZP2077]
ATGCTATCGGCGCCCACGCCGCTGGCTGAAAACCACGATCTCGAACTTTTCCAAAGTGGTACCGAAAGCCTCGACCAATG
GCTTCGCCGCAGGGCACGGGCCAACCAGGTCGGTGGCGCGTCGCGAACTTATGTGGTGGCGGAAGGCGCACGCGTTGTCG
GCTACTACTGTCTGTCTTCGGGCGGCCTTGATCTCACGTCGGCGCCTGGCCCCATCCGTCGCAACATACCGGATCCCATC
CCAATAGTTGTGCTCGGGCGCCTCGCCGTGGATGCAGGCTGGCAGGGAAAGGGGCTGGGCGCAGCGTTGCTGCAAGACGC
CGTTCTGCGCGCTGGCCATGCCGCAACGATCCTCGGGATTCGCGGCATTTTTGTTCATGCCGTATCGGATGAGGCGAAAG
CCTTTTATCAGCGCTACGGTTTTGCGGCTTCGCCGAAAAATCCGATGACCTTGGTGCTGTCGTTGAAAGGCCTGGTGAAA
GGGTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10620.15 Da
• Isoelectric Point: 7.2028

>AT113542 WP_172227191.1 NZ_CP033362:3263877-3264158 [Mesorhizobium sp. NZP2077]
MTKAAPETHAKPVNLRIREDMRILIDRAAKLRGKTRSDFMIDAAYRAAEDTLLNQALIKVDPESYRHYLDILDEPPGGEG
FGRLMNAPRPWQD

Nucleotide

• Length: 282 bp

>AT113542 NZ_CP033362:3263877-3264158 [Mesorhizobium sp. NZP2077]
ATGACGAAGGCCGCACCTGAGACCCATGCCAAGCCCGTCAATCTGCGCATCCGAGAGGACATGCGCATACTGATTGACCG
TGCAGCGAAATTACGCGGAAAAACCCGTTCCGACTTCATGATCGATGCCGCTTACCGTGCAGCCGAGGACACGTTGCTAA
ATCAAGCCCTGATCAAGGTGGATCCCGAGAGTTACCGGCATTATCTGGACATCCTTGATGAACCGCCAGGCGGGGAAGGT
TTTGGGCGCCTTATGAACGCGCCCAGGCCATGGCAGGACTGA