113540

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 6107201..6108912 Replicon chromosome
Accession NZ_CP033361
Organism Mesorhizobium erdmanii strain NZP2014

Toxin (Protein)


Gene name pmenT Uniprot ID A0A6M7UT03
Locus tag EB233_RS29360 Protein ID WP_064987309.1
Coordinates 6108280..6108912 (-) Length 211 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A6M7UT39
Locus tag EB233_RS29355 Protein ID WP_064987310.1
Coordinates 6107201..6108280 (-) Length 360 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB233_RS29330 6102884..6105070 - 2187 WP_064987314.1 tetratricopeptide repeat protein -
EB233_RS29335 6105540..6105860 - 321 WP_064987312.1 hypothetical protein -
EB233_RS29340 6105826..6106146 - 321 WP_081288669.1 helix-turn-helix domain-containing protein -
EB233_RS29345 6106287..6106928 + 642 Protein_5805 transposase -
EB233_RS29350 6106971..6107201 - 231 WP_064987311.1 RNA-binding protein -
EB233_RS29355 6107201..6108280 - 1080 WP_064987310.1 macro domain-containing protein Antitoxin
EB233_RS29360 6108280..6108912 - 633 WP_064987309.1 DUF4433 domain-containing protein Toxin
EB233_RS29365 6109197..6110230 - 1034 Protein_5809 IS110 family transposase -
EB233_RS29370 6110307..6110606 + 300 Protein_5810 IS110 family transposase -
EB233_RS29375 6110608..6110959 + 352 Protein_5811 IS630 family transposase -
EB233_RS29380 6110989..6111126 - 138 Protein_5812 transposase -
EB233_RS29385 6111628..6112596 - 969 WP_064987308.1 omptin family outer membrane protease -
EB233_RS29390 6112744..6112898 + 155 Protein_5814 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 6101938..6228940 127002
- flank IS/Tn - - 6106404..6106928 524


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(13-209)

Antitoxin

Macro domain

(18-132)


Sequences

Toxin        


Download         Length: 211 a.a.        Molecular weight: 23899.77 Da        Isoelectric Point: 8.2439

>T113540 WP_064987309.1 NZ_CP033361:c6108912-6108280 [Mesorhizobium erdmanii]
MSKDLNPEKALIFRIVHRDNIGPVLADGCHCRATMEGKKYIEIGNPELIQKRADKAVPIDPGGTLSDYVPFYFTPYSPML
YNIKTGYNGIKQRSMRDIVILVSSLHHLAKLEVPFVFTDRHAYLKLAQFSDDLADLNWIIWLTLQARDFKKDDIDKFEKY
QAEALVYKHVPLTALLGIVCYDENAKAEIEAAAAKAGAKVKVLAERRWFL

Download         Length: 633 bp

>T113540 NZ_CP033361:c6108912-6108280 [Mesorhizobium erdmanii]
ATGTCCAAAGACCTCAACCCAGAGAAAGCGCTCATTTTTCGGATTGTCCACAGGGACAATATCGGACCAGTGCTGGCTGA
TGGTTGCCACTGCCGCGCAACCATGGAAGGCAAGAAATATATCGAGATCGGGAATCCGGAGCTGATCCAGAAGCGCGCTG
ACAAGGCGGTTCCGATCGATCCGGGCGGCACTTTGAGCGACTACGTTCCGTTCTATTTCACACCCTATAGCCCGATGCTC
TACAACATCAAGACGGGTTACAACGGCATCAAGCAGCGCTCGATGCGAGACATCGTGATCCTCGTATCTTCGCTGCATCA
CCTGGCCAAGTTAGAGGTTCCGTTTGTGTTTACCGACCGGCACGCCTATCTGAAGTTGGCGCAGTTCTCGGACGATTTGG
CCGATCTGAACTGGATTATCTGGCTGACCCTGCAAGCGCGCGATTTCAAGAAAGATGACATCGACAAGTTCGAGAAATAT
CAGGCTGAAGCGCTTGTATATAAGCACGTTCCGCTCACTGCCCTGCTTGGCATCGTTTGCTATGACGAAAACGCAAAAGC
CGAGATCGAGGCTGCCGCCGCGAAAGCAGGCGCTAAGGTGAAGGTCCTGGCAGAAAGGAGATGGTTCCTCTGA

Antitoxin


Download         Length: 360 a.a.        Molecular weight: 40595.74 Da        Isoelectric Point: 6.2712

>AT113540 WP_064987310.1 NZ_CP033361:c6108280-6107201 [Mesorhizobium erdmanii]
MVKFVRGNLLEADTEALVNTVNTVGVMGKGVALMFKEAFPENFKLYEAACEDKRVRLGEMFVTERNDLYGPKWIINFPTK
AHWRYPSKIEWIAKGLDDLKAVVEHNNIKSIAIPPLGAGNGGLDWPEVREIILSKLDSLNDVEVLVYEPTRQYQNVAKRA
GVDKLTPARALIAELVRRYCILGIQCSLLEVQKLGYFVERFANREGLDEMKFEFGANKYGPYSDKMKHMLNALDGSYLHC
DKRLGDAGPFEPIHFDDERKERVSAYFSTAEAKPYRSALEATSKLIDGFESPLGMELLATVDWLMRHEKVAPQVNEIRAA
LPKWPGGEDAAERKLRLFDDRIIKIALDALKENSLVQAS

Download         Length: 1080 bp

>AT113540 NZ_CP033361:c6108280-6107201 [Mesorhizobium erdmanii]
ATGGTCAAGTTTGTCCGTGGCAACCTACTTGAAGCTGACACTGAAGCTCTGGTGAACACCGTCAACACCGTCGGCGTGAT
GGGGAAGGGCGTCGCCTTGATGTTCAAAGAGGCATTCCCCGAAAACTTCAAGCTTTACGAAGCCGCTTGTGAGGATAAGC
GCGTGCGGCTTGGCGAGATGTTCGTTACGGAGCGGAACGACCTCTATGGCCCGAAATGGATAATCAACTTTCCCACTAAG
GCGCACTGGCGATATCCATCTAAGATCGAATGGATAGCAAAGGGCTTGGACGATCTTAAAGCTGTCGTGGAACATAACAA
CATCAAATCCATAGCTATTCCACCTTTGGGTGCTGGCAACGGCGGCTTAGATTGGCCTGAAGTTCGCGAGATAATCCTGT
CTAAGCTTGATTCGCTAAATGATGTCGAAGTTCTTGTTTACGAGCCGACCCGACAATACCAGAACGTGGCCAAGCGTGCC
GGCGTAGATAAGTTGACTCCAGCGCGAGCGCTGATAGCTGAACTTGTTAGGAGATATTGTATTCTTGGAATCCAGTGCTC
GTTGCTGGAAGTTCAGAAACTTGGCTATTTCGTGGAGAGATTCGCGAACCGAGAGGGCTTGGATGAGATGAAATTCGAAT
TCGGAGCCAATAAATATGGGCCGTATTCGGACAAGATGAAGCATATGCTGAATGCTCTGGATGGAAGCTATTTACATTGC
GATAAGCGACTTGGTGATGCCGGCCCCTTTGAGCCAATTCATTTTGATGATGAGCGAAAGGAACGCGTCTCTGCCTACTT
TTCTACCGCTGAAGCAAAGCCATATCGATCAGCTTTGGAGGCCACCAGCAAGCTTATCGACGGCTTCGAGTCTCCCCTGG
GGATGGAGTTGCTTGCGACCGTCGATTGGTTGATGCGCCACGAAAAGGTTGCCCCCCAAGTGAATGAGATCAGGGCTGCC
CTGCCCAAATGGCCCGGTGGCGAGGATGCGGCTGAGCGGAAGTTGCGGCTGTTTGATGATCGCATCATCAAGATAGCCCT
GGATGCTTTGAAGGAAAACTCTTTGGTGCAGGCCAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6M7UT03

Antitoxin

Source ID Structure
AlphaFold DB A0A6M7UT39

References