Detailed information of TA system TA113531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 79389..79911
• Replicon: plasmid pCFSA664-1
• Accession: NZ_CP033353
• Organism: Salmonella enterica subsp. enterica strain CFSA664

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0QMR1
• Locus tag: EB780_RS02605
• Protein ID: WP_000220561.1
• Coordinates: 79630..79911 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: EB780_RS02600
• Protein ID: WP_000121743.1
• Coordinates: 79389..79640 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB780_RS02575	74980..75684	+	705	WP_032193599.1	replication initiation protein	-
EB780_RS02585	76500..77303	-	804	Protein_84	glutathione synthase	-
EB780_RS02590	77368..77631	+	264	Protein_85	IS6 family transposase	-
EB780_RS02595	77696..78400	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
EB780_RS02600	79389..79640	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
EB780_RS02605	79630..79911	+	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EB780_RS02610	80150..80242	-	93	Protein_89	IS6 family transposase	-
EB780_RS02615	80408..81607	-	1200	WP_000948429.1	IS91 family transposase	-
EB780_RS02620	81617..81805	-	189	WP_000957857.1	hypothetical protein	-
EB780_RS02625	81925..83355	+	1431	Protein_92	IS4-like element IS50R family transposase	-
EB780_RS02630	83384..84178	+	795	WP_000572405.1	aminoglycoside O-phosphotransferase APH(3')-IIa	-
EB780_RS02635	84199..84435	+	237	Protein_94	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA17 / aadA5 / oqxB / oqxA / armA / aph(3')-IIa / mph(A) / tet(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / floR	-	1..255327	255327
	-	inside	IScluster/Tn	aph(3')-IIa / mph(A)	-	74246..89146	14900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11023.86 Da
• Isoelectric Point: 10.5938

>T113531 WP_000220561.1 NZ_CP033353:79630-79911 [Salmonella enterica subsp. enterica]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T113531 NZ_CP033353:79630-79911 [Salmonella enterica subsp. enterica]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT113531 WP_000121743.1 NZ_CP033353:79389-79640 [Salmonella enterica subsp. enterica]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT113531 NZ_CP033353:79389-79640 [Salmonella enterica subsp. enterica]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A3S5I302

Antitoxin

• AlphaFold DB: A0A3S5I301