Detailed information of TA system TA113526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 29688..30213
• Replicon: plasmid pCFSAN076214_2
• Accession: NZ_CP033342
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain CFSAN076214

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: EAE33_RS23970
• Protein ID: WP_001159863.1
• Coordinates: 29908..30213 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: EAE33_RS23965
• Protein ID: WP_000813641.1
• Coordinates: 29688..29906 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAE33_RS23935	25302..25688	+	387	WP_000751876.1	YgiW/YdeI family stress tolerance OB fold protein	-
EAE33_RS23940	25741..25902	+	162	WP_015059604.1	hypothetical protein	-
EAE33_RS23945	26229..27218	-	990	WP_000461382.1	RepB family plasmid replication initiator protein	-
EAE33_RS23950	27712..28007	-	296	Protein_43	cytoplasmic protein	-
EAE33_RS24330	28019..28447	+	429	Protein_44	hypothetical protein	-
EAE33_RS23960	28491..29012	-	522	WP_077681952.1	hypothetical protein	-
EAE33_RS23965	29688..29906	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
EAE33_RS23970	29908..30213	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
EAE33_RS23975	30215..30505	+	291	WP_001266176.1	hypothetical protein	-
EAE33_RS23980	30502..31023	+	522	WP_000198608.1	hypothetical protein	-
EAE33_RS23985	31058..31840	+	783	WP_000082169.1	site-specific integrase	-
EAE33_RS23990	31849..32562	+	714	WP_000545756.1	EAL domain-containing protein	-
EAE33_RS23995	32566..33075	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
EAE33_RS24000	33069..33554	+	486	WP_000905606.1	membrane protein	-
EAE33_RS24005	33831..34118	-	288	WP_071530243.1	hypothetical protein	-
EAE33_RS24010	34274..34834	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56636	56636

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-99)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T113526 WP_001159863.1 NZ_CP033342:29908-30213 [Salmonella enterica subsp. enterica serovar Enteritidis]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T113526 NZ_CP033342:29908-30213 [Salmonella enterica subsp. enterica serovar Enteritidis]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT113526 WP_000813641.1 NZ_CP033342:29688-29906 [Salmonella enterica subsp. enterica serovar Enteritidis]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT113526 NZ_CP033342:29688-29906 [Salmonella enterica subsp. enterica serovar Enteritidis]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6