Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 6428290..6430001 Replicon chromosome
Accession NZ_CP033334
Organism Mesorhizobium loti strain NZP2042

Toxin (Protein)


Gene name pmenT Uniprot ID A0A6M7UT03
Locus tag EB815_RS31255 Protein ID WP_064987309.1
Coordinates 6429369..6430001 (-) Length 211 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A6M7UT39
Locus tag EB815_RS31250 Protein ID WP_064987310.1
Coordinates 6428290..6429369 (-) Length 360 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB815_RS31225 6423973..6426159 - 2187 WP_065005171.1 tetratricopeptide repeat protein -
EB815_RS31230 6426629..6426949 - 321 WP_064987312.1 hypothetical protein -
EB815_RS31235 6426915..6427235 - 321 WP_081288669.1 helix-turn-helix domain-containing protein -
EB815_RS31240 6427376..6428017 + 642 Protein_6180 transposase -
EB815_RS31245 6428060..6428290 - 231 WP_064987311.1 RNA-binding protein -
EB815_RS31250 6428290..6429369 - 1080 WP_064987310.1 macro domain-containing protein Antitoxin
EB815_RS31255 6429369..6430001 - 633 WP_064987309.1 DUF4433 domain-containing protein Toxin
EB815_RS31260 6430286..6431319 - 1034 Protein_6184 IS110 family transposase -
EB815_RS31265 6431396..6431695 + 300 Protein_6185 IS110 family transposase -
EB815_RS31270 6431697..6432048 + 352 Protein_6186 IS630 family transposase -
EB815_RS31275 6432078..6432215 - 138 Protein_6187 transposase -
EB815_RS31280 6432717..6433685 - 969 WP_064987308.1 omptin family outer membrane protease -
EB815_RS31285 6433833..6433987 + 155 Protein_6189 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 6423973..6441782 17809
- flank IS/Tn - - 6427493..6428017 524


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(13-209)

Antitoxin

Macro domain

(18-132)


Sequences

Toxin        


Download         Length: 211 a.a.        Molecular weight: 23899.77 Da        Isoelectric Point: 8.2439

>T113519 WP_064987309.1 NZ_CP033334:c6430001-6429369 [Mesorhizobium loti]
MSKDLNPEKALIFRIVHRDNIGPVLADGCHCRATMEGKKYIEIGNPELIQKRADKAVPIDPGGTLSDYVPFYFTPYSPML
YNIKTGYNGIKQRSMRDIVILVSSLHHLAKLEVPFVFTDRHAYLKLAQFSDDLADLNWIIWLTLQARDFKKDDIDKFEKY
QAEALVYKHVPLTALLGIVCYDENAKAEIEAAAAKAGAKVKVLAERRWFL

Download         Length: 633 bp

>T113519 NZ_CP033334:c6430001-6429369 [Mesorhizobium loti]
ATGTCCAAAGACCTCAACCCAGAGAAAGCGCTCATTTTTCGGATTGTCCACAGGGACAATATCGGACCAGTGCTGGCTGA
TGGTTGCCACTGCCGCGCAACCATGGAAGGCAAGAAATATATCGAGATCGGGAATCCGGAGCTGATCCAGAAGCGCGCTG
ACAAGGCGGTTCCGATCGATCCGGGCGGCACTTTGAGCGACTACGTTCCGTTCTATTTCACACCCTATAGCCCGATGCTC
TACAACATCAAGACGGGTTACAACGGCATCAAGCAGCGCTCGATGCGAGACATCGTGATCCTCGTATCTTCGCTGCATCA
CCTGGCCAAGTTAGAGGTTCCGTTTGTGTTTACCGACCGGCACGCCTATCTGAAGTTGGCGCAGTTCTCGGACGATTTGG
CCGATCTGAACTGGATTATCTGGCTGACCCTGCAAGCGCGCGATTTCAAGAAAGATGACATCGACAAGTTCGAGAAATAT
CAGGCTGAAGCGCTTGTATATAAGCACGTTCCGCTCACTGCCCTGCTTGGCATCGTTTGCTATGACGAAAACGCAAAAGC
CGAGATCGAGGCTGCCGCCGCGAAAGCAGGCGCTAAGGTGAAGGTCCTGGCAGAAAGGAGATGGTTCCTCTGA

Antitoxin


Download         Length: 360 a.a.        Molecular weight: 40595.74 Da        Isoelectric Point: 6.2712

>AT113519 WP_064987310.1 NZ_CP033334:c6429369-6428290 [Mesorhizobium loti]
MVKFVRGNLLEADTEALVNTVNTVGVMGKGVALMFKEAFPENFKLYEAACEDKRVRLGEMFVTERNDLYGPKWIINFPTK
AHWRYPSKIEWIAKGLDDLKAVVEHNNIKSIAIPPLGAGNGGLDWPEVREIILSKLDSLNDVEVLVYEPTRQYQNVAKRA
GVDKLTPARALIAELVRRYCILGIQCSLLEVQKLGYFVERFANREGLDEMKFEFGANKYGPYSDKMKHMLNALDGSYLHC
DKRLGDAGPFEPIHFDDERKERVSAYFSTAEAKPYRSALEATSKLIDGFESPLGMELLATVDWLMRHEKVAPQVNEIRAA
LPKWPGGEDAAERKLRLFDDRIIKIALDALKENSLVQAS

Download         Length: 1080 bp

>AT113519 NZ_CP033334:c6429369-6428290 [Mesorhizobium loti]
ATGGTCAAGTTTGTCCGTGGCAACCTACTTGAAGCTGACACTGAAGCTCTGGTGAACACCGTCAACACCGTCGGCGTGAT
GGGGAAGGGCGTCGCCTTGATGTTCAAAGAGGCATTCCCCGAAAACTTCAAGCTTTACGAAGCCGCTTGTGAGGATAAGC
GCGTGCGGCTTGGCGAGATGTTCGTTACGGAGCGGAACGACCTCTATGGCCCGAAATGGATAATCAACTTTCCCACTAAG
GCGCACTGGCGATATCCATCTAAGATCGAATGGATAGCAAAGGGCTTGGACGATCTTAAAGCTGTCGTGGAACATAACAA
CATCAAATCCATAGCTATTCCACCTTTGGGTGCTGGCAACGGCGGCTTAGATTGGCCTGAAGTTCGCGAGATAATCCTGT
CTAAGCTTGATTCGCTAAATGATGTCGAAGTTCTTGTTTACGAGCCGACCCGACAATACCAGAACGTGGCCAAGCGTGCC
GGCGTAGATAAGTTGACTCCAGCGCGAGCGCTGATAGCTGAACTTGTTAGGAGATATTGTATTCTTGGAATCCAGTGCTC
GTTGCTGGAAGTTCAGAAACTTGGCTATTTCGTGGAGAGATTCGCGAACCGAGAGGGCTTGGATGAGATGAAATTCGAAT
TCGGAGCCAATAAATATGGACCGTATTCGGACAAGATGAAGCATATGCTGAATGCTCTGGATGGAAGCTATTTACATTGC
GATAAGCGACTTGGTGATGCCGGCCCCTTTGAGCCAATTCATTTTGATGATGAGCGAAAGGAACGCGTCTCTGCCTACTT
TTCTACCGCTGAAGCAAAGCCATATCGATCAGCTTTGGAGGCCACCAGCAAGCTTATCGACGGCTTCGAGTCTCCCCTGG
GGATGGAGTTGCTTGCGACCGTCGATTGGTTGATGCGCCACGAAAAGGTTGCCCCCCAAGTGAATGAGATCAGGGCTGCC
CTGCCCAAATGGCCCGGTGGCGAGGATGCGGCTGAGCGGAAGTTGCGGCTGTTTGATGATCGCATCATCAAGATAGCCCT
GGATGCTTTGAAGGAAAACTCTTTGGTGCAGGCCAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6M7UT03

Antitoxin

Source ID Structure
AlphaFold DB A0A6M7UT39

References