Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 3686768..3687441 Replicon chromosome
Accession NZ_CP033334
Organism Mesorhizobium loti strain NZP2042

Toxin (Protein)


Gene name vapC Uniprot ID A0A0Q8KMJ8
Locus tag EB815_RS18005 Protein ID WP_056571852.1
Coordinates 3687043..3687441 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0Q8KL58
Locus tag EB815_RS18000 Protein ID WP_056571849.1
Coordinates 3686768..3687043 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB815_RS17975 3681987..3682973 + 987 WP_155772460.1 ABC transporter permease -
EB815_RS17980 3683090..3683638 + 549 WP_056571843.1 gluconokinase -
EB815_RS17985 3683633..3684769 - 1137 WP_056572160.1 hypothetical protein -
EB815_RS17990 3684869..3685318 - 450 WP_027029809.1 Rrf2 family transcriptional regulator -
EB815_RS17995 3685557..3686543 - 987 WP_056571846.1 sulfate ABC transporter substrate-binding protein -
EB815_RS18000 3686768..3687043 + 276 WP_056571849.1 antitoxin Antitoxin
EB815_RS18005 3687043..3687441 + 399 WP_056571852.1 type II toxin-antitoxin system VapC family toxin Toxin
EB815_RS18010 3687458..3688498 - 1041 WP_056571856.1 sulfate/molybdate ABC transporter ATP-binding protein -
EB815_RS18015 3688512..3689396 - 885 WP_056571857.1 sulfate ABC transporter permease subunit CysW -
EB815_RS18020 3689389..3690240 - 852 WP_056571860.1 sulfate ABC transporter permease subunit CysT -
EB815_RS18025 3690324..3691352 - 1029 WP_056571863.1 sulfate ABC transporter substrate-binding protein -
EB815_RS18030 3691564..3692286 - 723 WP_056571866.1 M15 family metallopeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

SpoVT-AbrB domain

(18-56)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14209.55 Da        Isoelectric Point: 6.4580

>T113518 WP_056571852.1 NZ_CP033334:3687043-3687441 [Mesorhizobium loti]
MRFMLDTNVISDMIRNPAGKAASAMVREGDAAVCTSIVVASELRYGCARKGSAKLLKKVEDLLAEIPVLPLDVSVDAEYG
ALRAELEAADQPIGHNDLFIAAHACVLGTTLVTANVGKFARIRKLKVENWLE

Download         Length: 399 bp

>T113518 NZ_CP033334:3687043-3687441 [Mesorhizobium loti]
ATGCGGTTCATGTTGGATACAAACGTCATTAGCGACATGATCCGAAACCCGGCCGGCAAGGCTGCCAGTGCAATGGTGCG
CGAAGGTGACGCCGCTGTTTGCACCAGCATCGTCGTCGCCTCTGAATTGCGATATGGGTGCGCCAGGAAGGGATCGGCCA
AGCTGCTCAAGAAGGTCGAGGATCTGCTGGCCGAGATCCCTGTATTGCCGCTCGACGTATCGGTGGACGCTGAATACGGC
GCGCTTCGCGCCGAGTTGGAGGCCGCTGACCAACCCATTGGCCACAATGACCTTTTTATTGCCGCTCACGCCTGCGTGTT
GGGCACTACATTGGTGACGGCCAATGTGGGGAAGTTCGCCCGGATACGGAAGCTGAAGGTCGAGAACTGGCTGGAATAG

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10155.60 Da        Isoelectric Point: 5.7252

>AT113518 WP_056571849.1 NZ_CP033334:3686768-3687043 [Mesorhizobium loti]
VPQHRHTTASPPKEAKLFRNNRSQAVRIPVEFELPGDKVLISREGDRLVIEPLRKPGLAALLAQWAKEPPLGPEDDFPGI
RDAPVEAEDIF

Download         Length: 276 bp

>AT113518 NZ_CP033334:3686768-3687043 [Mesorhizobium loti]
GTGCCACAGCACCGGCATACAACCGCGTCGCCTCCGAAGGAGGCCAAGCTCTTCCGCAACAACCGCAGCCAGGCGGTGCG
TATTCCCGTCGAATTCGAGCTCCCCGGTGACAAGGTTCTGATCAGCCGCGAAGGCGACCGCCTTGTCATCGAGCCGCTCC
GCAAACCAGGCCTTGCCGCGCTGCTCGCCCAATGGGCGAAAGAGCCACCGCTTGGTCCTGAGGACGACTTTCCCGGGATC
AGGGATGCGCCTGTTGAAGCCGAGGACATTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q8KMJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0Q8KL58

References