Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 544505..545187 Replicon chromosome
Accession NZ_CP033334
Organism Mesorhizobium loti strain NZP2042

Toxin (Protein)


Gene name vapC Uniprot ID A0A6M7TTC2
Locus tag EB815_RS02485 Protein ID WP_065005419.1
Coordinates 544765..545187 (+) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0Q8KUW5
Locus tag EB815_RS02480 Protein ID WP_056570294.1
Coordinates 544505..544768 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB815_RS02455 539587..540528 + 942 WP_056568850.1 sugar ABC transporter substrate-binding protein -
EB815_RS02460 540638..541714 + 1077 WP_056568846.1 ABC transporter permease -
EB815_RS02465 541717..542484 + 768 WP_027040720.1 sugar ABC transporter ATP-binding protein -
EB815_RS02470 542604..543599 + 996 WP_056568843.1 inositol 2-dehydrogenase -
EB815_RS02475 543626..544420 + 795 WP_056568841.1 SDR family oxidoreductase -
EB815_RS02480 544505..544768 + 264 WP_056570294.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EB815_RS02485 544765..545187 + 423 WP_065005419.1 type II toxin-antitoxin system VapC family toxin Toxin
EB815_RS02490 545637..546728 + 1092 WP_056570292.1 3-deoxy-7-phosphoheptulonate synthase -
EB815_RS02495 546776..547411 - 636 WP_065005420.1 LysE family translocator -
EB815_RS02500 547541..548236 + 696 WP_056568830.1 YafY family transcriptional regulator -
EB815_RS02505 548249..549358 - 1110 WP_056568828.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(8-70)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15632.99 Da        Isoelectric Point: 4.9995

>T113516 WP_065005419.1 NZ_CP033334:544765-545187 [Mesorhizobium loti]
VRLLLDTNVLSEVTRPGPDARVLDWLDGLDEDRSFISVVSIAEIRRGVALMDEGRKRKALAEWLARDLPQRFEQRILPVD
EPVALAWGDLIGLAKRRGRGLSSMDGLIAATAIAQRLTLVTRNTRDFEGFGIELLDPWTA

Download         Length: 423 bp

>T113516 NZ_CP033334:544765-545187 [Mesorhizobium loti]
GTGAGGCTGCTGCTCGATACGAATGTCCTGTCCGAGGTGACAAGGCCGGGGCCAGACGCGCGCGTTCTGGACTGGCTGGA
CGGGCTTGATGAGGATCGCTCATTCATCAGTGTCGTGTCGATCGCCGAGATCCGGCGTGGCGTCGCCCTCATGGACGAGG
GCCGGAAACGCAAGGCACTGGCCGAATGGCTCGCCCGAGACCTGCCGCAGCGTTTCGAGCAAAGGATTTTGCCTGTGGAC
GAGCCGGTTGCCCTGGCCTGGGGCGACCTTATAGGCCTTGCGAAGCGTCGCGGTCGCGGCCTGTCGTCAATGGACGGGCT
GATCGCAGCCACGGCGATTGCGCAGCGGCTAACCTTGGTAACGCGCAATACCAGGGATTTCGAAGGCTTTGGGATAGAAC
TCCTCGATCCCTGGACGGCATGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9640.00 Da        Isoelectric Point: 10.0862

>AT113516 WP_056570294.1 NZ_CP033334:544505-544768 [Mesorhizobium loti]
MLPVAKDTSWTVAGAKARLSEVIERAQSSPQTITKNGKPSVVVVSAEEWQRKTARKGTLAQFLLESPLRGADLDLERQRD
EPRDLPL

Download         Length: 264 bp

>AT113516 NZ_CP033334:544505-544768 [Mesorhizobium loti]
ATGCTGCCTGTGGCCAAAGATACGAGCTGGACGGTCGCTGGGGCAAAGGCTCGGCTCTCGGAGGTTATCGAGCGCGCGCA
GTCTTCTCCGCAGACGATCACCAAAAACGGCAAACCAAGCGTGGTTGTCGTCTCGGCCGAGGAATGGCAGCGAAAGACCG
CGCGCAAGGGTACGCTTGCGCAGTTCCTGCTGGAGTCACCGCTGCGCGGCGCCGATCTCGACCTCGAGCGCCAGCGTGAC
GAACCGCGTGACCTGCCGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6M7TTC2

Antitoxin

Source ID Structure
AlphaFold DB A0A0Q8KUW5

References