Detailed information of TA system TA113507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 36453..37251
• Replicon: plasmid pTB212
• Accession: NZ_CP033252
• Organism: Escherichia coli strain ECCHD184

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: D3W51_RS24315
• Protein ID: WP_033870689.1
• Coordinates: 36730..37251 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: D3W51_RS24310
• Protein ID: WP_001711191.1
• Coordinates: 36453..36722 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3W51_RS24285	31877..33217	-	1341	WP_023135655.1	AAA family ATPase	-
D3W51_RS24290	33278..34003	-	726	WP_087904723.1	hypothetical protein	-
D3W51_RS24295	34286..35053	+	768	WP_033870691.1	hypothetical protein	-
D3W51_RS24300	35106..35459	-	354	WP_160378290.1	hypothetical protein	-
D3W51_RS24305	35465..36133	-	669	WP_000161228.1	AAA family ATPase	-
D3W51_RS24310	36453..36722	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
D3W51_RS24315	36730..37251	+	522	WP_033870689.1	GNAT family N-acetyltransferase	Toxin
D3W51_RS24325	37420..37671	-	252	WP_063122787.1	hypothetical protein	-
D3W51_RS24330	37674..38366	-	693	WP_024172707.1	membrane protein	-
D3W51_RS24335	38380..38703	-	324	WP_001711185.1	hypothetical protein	-
D3W51_RS24340	38837..39418	-	582	WP_063122786.1	tail fiber assembly protein	-
D3W51_RS24345	39427..40329	-	903	WP_001711183.1	macro domain-containing protein	-
D3W51_RS24350	40326..42122	-	1797	WP_063122796.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110417	110417

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19395.22 Da
• Isoelectric Point: 8.2535

>T113507 WP_033870689.1 NZ_CP033252:36730-37251 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKENLPSKSQQ
KKVPYRHVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T113507 NZ_CP033252:36730-37251 [Escherichia coli]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAAGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAAACTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGCATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAGGGGCT
TGGCTCAATGCTTGTAACTCACGCAATGCGTGTTGTGTACAATGCATCTCTTGCGGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTGAACGATAAAGCCAGGGCGTTTTATAAAAGTTTGGGCTTTATCCAGCTGGTTGGTAACAACGATCGTTCTTTG
TTCTATCCAACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT113507 WP_001711191.1 NZ_CP033252:36453-36722 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT113507 NZ_CP033252:36453-36722 [Escherichia coli]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACCCAGTTCATGGTTAGCAGTGCATCCGAACGTGCCGTGAAGGTGATAGAGCAACACCGTAGAC
TGGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2