Detailed information of TA system TA113506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 29873..30471
• Replicon: plasmid pTB212
• Accession: NZ_CP033252
• Organism: Escherichia coli strain ECCHD184

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: D3W51_RS24270
• Protein ID: WP_053291281.1
• Coordinates: 30094..30471 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: D3W51_RS24265
• Protein ID: WP_001603498.1
• Coordinates: 29873..30094 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3W51_RS24245	24879..25427	+	549	WP_122224844.1	fimbrial protein YehD	-
D3W51_RS24250	25483..26163	+	681	WP_032187672.1	fimbrial assembly chaperone	-
D3W51_RS24255	26181..28667	+	2487	WP_122224845.1	fimbria/pilus outer membrane usher protein	-
D3W51_RS24260	28678..29688	+	1011	WP_087905101.1	fimbrial protein	-
D3W51_RS24265	29873..30094	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D3W51_RS24270	30094..30471	+	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
D3W51_RS24280	30605..31720	-	1116	WP_024144822.1	hypothetical protein	-
D3W51_RS24285	31877..33217	-	1341	WP_023135655.1	AAA family ATPase	-
D3W51_RS24290	33278..34003	-	726	WP_087904723.1	hypothetical protein	-
D3W51_RS24295	34286..35053	+	768	WP_033870691.1	hypothetical protein	-
D3W51_RS24300	35106..35459	-	354	WP_160378290.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110417	110417

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T113506 WP_053291281.1 NZ_CP033252:30094-30471 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T113506 NZ_CP033252:30094-30471 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGCC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT113506 WP_001603498.1 NZ_CP033252:29873-30094 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT113506 NZ_CP033252:29873-30094 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0