Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 93936..94537 Replicon plasmid pTB211
Accession NZ_CP033251
Organism Escherichia coli strain ECCHD184

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag D3W51_RS23730 Protein ID WP_001216034.1
Coordinates 94157..94537 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag D3W51_RS23725 Protein ID WP_001190712.1
Coordinates 93936..94157 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3W51_RS23715 90962..92200 - 1239 WP_038997080.1 restriction endonuclease subunit S -
D3W51_RS23720 92197..93753 - 1557 WP_038997084.1 type I restriction-modification system subunit M -
D3W51_RS23725 93936..94157 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D3W51_RS23730 94157..94537 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
D3W51_RS23735 94542..94721 + 180 WP_038997082.1 hypothetical protein -
D3W51_RS23740 94749..95213 + 465 Protein_97 hypothetical protein -
D3W51_RS23745 95227..95924 - 698 Protein_98 IS1 family transposase -
D3W51_RS23750 95980..96342 + 363 Protein_99 phage antirepressor KilAC domain-containing protein -
D3W51_RS23755 96375..97226 - 852 WP_000611664.1 hypothetical protein -
D3W51_RS23760 97337..97546 - 210 WP_000874156.1 hypothetical protein -
D3W51_RS23770 97717..97962 + 246 Protein_102 IS1 family transposase -
D3W51_RS23775 98257..98954 - 698 Protein_103 IS1 family transposase -
D3W51_RS23780 99056..99379 - 324 WP_001175593.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / sul1 / qacE / aadA5 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / blaCTX-M-14 / fosA3 - 1..164062 164062


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T113504 WP_001216034.1 NZ_CP033251:94157-94537 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T113504 NZ_CP033251:94157-94537 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT113504 WP_001190712.1 NZ_CP033251:93936-94157 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT113504 NZ_CP033251:93936-94157 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References