Detailed information of TA system TA113499

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3741459..3742257
• Replicon: chromosome
• Accession: NZ_CP033250
• Organism: Escherichia coli strain ECCHD184

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A166U9J9
• Locus tag: D3W51_RS18185
• Protein ID: WP_000854695.1
• Coordinates: 3741459..3741836 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2U2UUE3
• Locus tag: D3W51_RS18190
• Protein ID: WP_016243360.1
• Coordinates: 3741883..3742257 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3W51_RS18155	3736737..3737693	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
D3W51_RS18160	3737744..3738082	+	339	Protein_3531	LysR family transcriptional regulator	-
D3W51_RS18165	3738420..3739739	+	1320	WP_000144689.1	site-specific integrase	-
D3W51_RS18170	3739832..3740680	-	849	WP_001280536.1	DUF4942 domain-containing protein	-
D3W51_RS18175	3740765..3740962	-	198	WP_000839228.1	DUF957 domain-containing protein	-
D3W51_RS18180	3740974..3741462	-	489	WP_000761710.1	hypothetical protein	-
D3W51_RS18185	3741459..3741836	-	378	WP_000854695.1	TA system toxin CbtA family protein	Toxin
D3W51_RS18190	3741883..3742257	-	375	WP_016243360.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
D3W51_RS18195	3742420..3742641	-	222	WP_000692307.1	DUF987 domain-containing protein	-
D3W51_RS18200	3742710..3743186	-	477	WP_001186773.1	RadC family protein	-
D3W51_RS18205	3743202..3743681	-	480	WP_000860084.1	antirestriction protein	-
D3W51_RS18210	3743763..3744581	-	819	WP_001241366.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3712071..3743681	31610

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14062.03 Da
• Isoelectric Point: 7.7531

>T113499 WP_000854695.1 NZ_CP033250:c3741836-3741459 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPGAKQ

Nucleotide

• Length: 378 bp

>T113499 NZ_CP033250:c3741836-3741459 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGCACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13810.66 Da
• Isoelectric Point: 7.0266

>AT113499 WP_016243360.1 NZ_CP033250:c3742257-3741883 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT113499 NZ_CP033250:c3742257-3741883 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGAGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A166U9J9

Antitoxin

• AlphaFold DB: A0A2U2UUE3