Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3555244..3555862 Replicon chromosome
Accession NZ_CP033250
Organism Escherichia coli strain ECCHD184

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag D3W51_RS17285 Protein ID WP_001291435.1
Coordinates 3555644..3555862 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag D3W51_RS17280 Protein ID WP_000344800.1
Coordinates 3555244..3555618 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3W51_RS17270 3550333..3551526 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
D3W51_RS17275 3551549..3554698 + 3150 WP_122224745.1 efflux RND transporter permease AcrB -
D3W51_RS17280 3555244..3555618 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
D3W51_RS17285 3555644..3555862 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
D3W51_RS17290 3556034..3556585 + 552 WP_000102564.1 maltose O-acetyltransferase -
D3W51_RS17295 3556701..3557171 + 471 WP_000136192.1 YlaC family protein -
D3W51_RS17300 3557335..3558885 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
D3W51_RS17305 3558927..3559280 - 354 WP_000878140.1 DUF1428 domain-containing protein -
D3W51_RS17315 3559659..3559970 + 312 WP_000409911.1 MGMT family protein -
D3W51_RS17320 3560001..3560573 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T113498 WP_001291435.1 NZ_CP033250:3555644-3555862 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T113498 NZ_CP033250:3555644-3555862 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT113498 WP_000344800.1 NZ_CP033250:3555244-3555618 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT113498 NZ_CP033250:3555244-3555618 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References