Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 854699..855353 Replicon chromosome
Accession NZ_CP033250
Organism Escherichia coli strain ECCHD184

Toxin (Protein)


Gene name cptA Uniprot ID A0A193LWV0
Locus tag D3W51_RS04145 Protein ID WP_065227581.1
Coordinates 854946..855353 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag D3W51_RS04140 Protein ID WP_000354046.1
Coordinates 854699..854965 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3W51_RS04115 849868..850611 + 744 WP_000951964.1 SDR family oxidoreductase -
D3W51_RS04120 850668..852101 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
D3W51_RS04125 852146..852457 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
D3W51_RS04130 852621..853280 + 660 WP_000250274.1 hemolysin III family protein -
D3W51_RS04135 853476..854456 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
D3W51_RS04140 854699..854965 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
D3W51_RS04145 854946..855353 + 408 WP_065227581.1 protein YgfX Toxin
D3W51_RS04150 855393..855914 - 522 WP_001055874.1 flavodoxin FldB -
D3W51_RS04155 856026..856922 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
D3W51_RS04160 856947..857657 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
D3W51_RS04165 857663..859396 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16001.93 Da        Isoelectric Point: 11.5202

>T113482 WP_065227581.1 NZ_CP033250:854946-855353 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQEAQR

Download         Length: 408 bp

>T113482 NZ_CP033250:854946-855353 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGGCGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT113482 WP_000354046.1 NZ_CP033250:854699-854965 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT113482 NZ_CP033250:854699-854965 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A193LWV0

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References