Detailed information of TA system TA113470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2019409..2020015
• Replicon: chromosome
• Accession: NZ_CP033241
• Organism: Saccharolobus solfataricus strain SUL120

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q97YS3
• Locus tag: SULZ_RS11335
• Protein ID: WP_009993017.1
• Coordinates: 2019623..2020015 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3G8ES43
• Locus tag: SULZ_RS11330
• Protein ID: WP_009993015.1
• Coordinates: 2019409..2019633 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SULZ_RS11305 (SULZ_11275)	2014557..2015687	+	1131	WP_009992604.1	aromatic/alkene monooxygenase hydroxylase subunit beta	-
SULZ_RS11310 (SULZ_11280)	2015684..2016145	+	462	WP_010923265.1	metal-sulfur cluster assembly factor	-
SULZ_RS11315 (SULZ_11285)	2016188..2017103	-	916	Protein_2190	IS110 family transposase	-
SULZ_RS11320 (SULZ_11290)	2017148..2018131	+	984	WP_010923242.1	IS630-like element ISC1048 family transposase	-
SULZ_RS11325 (SULZ_11295)	2018130..2019099	+	970	Protein_2192	IS110 family transposase	-
SULZ_RS11330 (SULZ_11300)	2019409..2019633	+	225	WP_009993015.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
SULZ_RS11335 (SULZ_11305)	2019623..2020015	+	393	WP_009993017.1	type II toxin-antitoxin system VapC family toxin	Toxin
SULZ_RS11340 (SULZ_11310)	2020458..2020936	-	479	Protein_2195	ISNCY family transposase	-
SULZ_RS11345 (SULZ_11315)	2021054..2022106	+	1053	WP_010923271.1	ISH3 family transposase	-
SULZ_RS11350 (SULZ_11320)	2022294..2022881	-	588	Protein_2197	ISNCY family transposase	-
SULZ_RS11355 (SULZ_11325)	2023315..2023656	-	342	WP_009990179.1	nucleotidyltransferase domain-containing protein	-
SULZ_RS11360 (SULZ_11330)	2023647..2023994	-	348	WP_014511635.1	HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2011132..2022881	11749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (8-124)

Antitoxin

• Post-segregation antitoxin CcdA (5-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 15117.81 Da
• Isoelectric Point: 9.3474

>T113470 WP_009993017.1 NZ_CP033241:2019623-2020015 [Saccharolobus solfataricus]
MKDKEFLLDASALYSLLDYVDKVDVKKIHVLTLTFYEVGNVIWKEYYIHKKVKDPITLSRLFYKLMRKFNVIEDSPLEGV
MRIAIERGLTYYNASYAYVAESLGLILVSNDKELIRKANAISLKDLIKSM

Nucleotide

• Length: 393 bp

>T113470 NZ_CP033241:2019623-2020015 [Saccharolobus solfataricus]
ATGAAAGATAAGGAATTCTTGCTCGATGCCTCAGCTTTGTATTCACTTTTAGATTACGTGGATAAGGTAGATGTTAAGAA
AATTCATGTACTTACCCTAACTTTTTATGAGGTAGGTAATGTCATATGGAAGGAGTATTATATACATAAAAAGGTTAAAG
ATCCTATAACCCTTTCGAGGCTTTTCTATAAATTAATGCGAAAATTTAACGTAATAGAGGATTCGCCTCTTGAGGGGGTA
ATGAGAATTGCCATAGAAAGGGGTTTAACTTACTATAACGCATCTTATGCGTATGTTGCTGAATCTTTAGGGCTTATCCT
AGTGTCTAACGATAAAGAGTTGATAAGAAAGGCTAACGCTATTTCGTTAAAGGATTTGATAAAAAGTATGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8840.20 Da
• Isoelectric Point: 5.0190

>AT113470 WP_009993015.1 NZ_CP033241:2019409-2019633 [Saccharolobus solfataricus]
MSDVISVRVKKELKKKAEELGINVREVVEKALEEAIREKEKEELKDMTMKIKELMRDVSEYDWVSTVRESRDER

Nucleotide

• Length: 225 bp

>AT113470 NZ_CP033241:2019409-2019633 [Saccharolobus solfataricus]
ATGTCAGACGTAATAAGCGTAAGAGTGAAGAAAGAGCTAAAGAAGAAGGCAGAGGAGTTAGGAATTAACGTTAGGGAAGT
TGTAGAAAAAGCTTTAGAGGAAGCCATAAGGGAGAAGGAGAAGGAAGAGCTTAAGGATATGACTATGAAAATTAAGGAAC
TAATGAGGGACGTAAGTGAATATGATTGGGTAAGTACTGTTAGGGAGAGTAGGGATGAAAGATAA

Structures

Toxin

• AlphaFold DB: A0A0E3GVD1

Antitoxin

• AlphaFold DB: A0A3G8ES43