Detailed information of TA system TA113469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 828417..829023
• Replicon: chromosome
• Accession: NZ_CP033241
• Organism: Saccharolobus solfataricus strain SUL120

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q97UE3
• Locus tag: SULZ_RS04400
• Protein ID: WP_009989090.1
• Coordinates: 828417..828821 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M9UBQ9
• Locus tag: SULZ_RS04405
• Protein ID: WP_009989091.1
• Coordinates: 828802..829023 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SULZ_RS04365 (SULZ_04375)	823919..824680	-	762	WP_009990867.1	ZIP family metal transporter	-
SULZ_RS04370 (SULZ_04380)	824828..825010	-	183	WP_009990866.1	hypothetical protein	-
SULZ_RS04380 (SULZ_04390)	825846..826625	+	780	WP_014511459.1	DMT family transporter	-
SULZ_RS04385 (SULZ_04395)	827063..827787	+	725	Protein_871	transposase	-
SULZ_RS04390 (SULZ_04400)	827785..827892	-	108	Protein_872	IS110 family transposase	-
SULZ_RS04395 (SULZ_04405)	827934..828221	+	288	WP_272898411.1	transposase	-
SULZ_RS04400 (SULZ_04410)	828417..828821	-	405	WP_009989090.1	PIN domain-containing protein	Toxin
SULZ_RS04405 (SULZ_04415)	828802..829023	-	222	WP_009989091.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SULZ_RS04410 (SULZ_04420)	829552..829752	+	201	WP_009989095.1	hypothetical protein	-
SULZ_RS04415 (SULZ_04425)	829806..830036	+	231	WP_009989096.1	EamA family transporter	-
SULZ_RS04420 (SULZ_04430)	830147..831418	+	1272	WP_009989097.1	MFS transporter	-
SULZ_RS04425 (SULZ_04435)	831537..832202	+	666	WP_009989098.1	helix-turn-helix domain-containing protein	-
SULZ_RS04430 (SULZ_04440)	832199..832585	-	387	WP_009989099.1	hypothetical protein	-
SULZ_RS04435 (SULZ_04445)	832582..833199	-	618	WP_009989100.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• SpoVT-AbrB domain (4-48)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15426.98 Da
• Isoelectric Point: 5.0272

>T113469 WP_009989090.1 NZ_CP033241:c828821-828417 [Saccharolobus solfataricus]
VEKAIVDTNVIIYDYVEDSEYHKRAEELLDSLDRWVIPVIVVHEIMWFLKGMKLEDKVDDVLAYIRSEKAEVVCDCTDNV
TDAIGVLNKEKLPLVSYKDMVILSHAIREKLPLATFDRKLSKIAKKYSVSLLQS

Nucleotide

• Length: 405 bp

>T113469 NZ_CP033241:c828821-828417 [Saccharolobus solfataricus]
GTGGAGAAGGCAATAGTTGACACTAACGTGATAATTTACGACTACGTTGAAGACTCCGAATATCATAAGAGGGCGGAAGA
GCTTCTCGATAGTTTGGATAGGTGGGTAATTCCCGTGATTGTAGTTCATGAGATCATGTGGTTCTTAAAGGGCATGAAAC
TTGAAGACAAGGTAGATGACGTTTTGGCTTATATTAGAAGTGAGAAAGCTGAGGTTGTCTGCGATTGTACAGATAACGTC
ACTGACGCTATAGGAGTGCTGAATAAGGAAAAGCTTCCCTTGGTCAGCTACAAAGATATGGTAATACTTTCTCACGCAAT
TAGAGAAAAATTGCCTTTAGCCACTTTTGATAGGAAACTCTCAAAGATAGCAAAGAAGTACTCCGTAAGTTTGCTACAGT
CGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8267.50 Da
• Isoelectric Point: 9.6908

>AT113469 WP_009989091.1 NZ_CP033241:c829023-828802 [Saccharolobus solfataricus]
MERVKVTRNYQITIPASARSKINIKEGDILEVYLNGDEIVLRKVKTERPRIRLGSKLSLGDIEEAIERGEGNS

Nucleotide

• Length: 222 bp

>AT113469 NZ_CP033241:c829023-828802 [Saccharolobus solfataricus]
ATGGAGAGGGTAAAGGTTACGAGAAATTACCAGATAACTATTCCAGCCTCGGCTAGGAGTAAGATTAATATAAAAGAAGG
AGATATACTTGAGGTTTACTTGAACGGTGACGAGATAGTACTTCGGAAGGTTAAGACTGAGAGGCCGAGGATAAGGCTAG
GCAGTAAACTCTCACTGGGAGATATTGAGGAGGCAATAGAGCGTGGAGAAGGCAATAGTTGA

Structures

Toxin

• AlphaFold DB: A0A0E3K068

Antitoxin

• AlphaFold DB: M9UBQ9