Detailed information of TA system TA113465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 2345261..2345908
• Replicon: chromosome
• Accession: NZ_CP033240
• Organism: Saccharolobus solfataricus strain SARC-O

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SULO_RS13035
• Protein ID: WP_009990357.1
• Coordinates: 2345261..2345533 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E3K976
• Locus tag: SULO_RS13040
• Protein ID: WP_009990356.1
• Coordinates: 2345648..2345908 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SULO_RS13010 (SULO_13040)	2340576..2341627	-	1052	Protein_2523	ISH3-like element ISC1359 family transposase	-
SULO_RS13015 (SULO_13045)	2341731..2342480	+	750	Protein_2524	GDP-mannose 4,6-dehydratase	-
SULO_RS13025 (SULO_13060)	2343791..2344738	-	948	WP_009990359.1	ATP/GTP-binding protein	-
SULO_RS13035 (SULO_13070)	2345261..2345533	-	273	WP_009990357.1	PIN domain-containing protein	Toxin
SULO_RS13040 (SULO_13075)	2345648..2345908	-	261	WP_009990356.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SULO_RS13045 (SULO_13080)	2346595..2346756	+	162	WP_009990355.1	hypothetical protein	-
SULO_RS13050 (SULO_13085)	2346858..2347316	+	459	WP_009990354.1	hypothetical protein	-
SULO_RS13055 (SULO_13090)	2348065..2348571	-	507	WP_014511696.1	thiosulfate:quinone oxidoreductase small subunit	-
SULO_RS13060 (SULO_13095)	2348577..2349119	-	543	WP_009990352.1	thiosulfate:quinone oxidoreductase large subunit	-
SULO_RS13065 (SULO_13100)	2349427..2350302	-	876	Protein_2532	transposase	-
SULO_RS13070 (SULO_13105)	2350663..2350896	+	234	WP_009993269.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (20-82)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10475.34 Da
• Isoelectric Point: 9.7534

>T113465 WP_009990357.1 NZ_CP033240:c2345533-2345261 [Saccharolobus solfataricus]
MRLFTLSKKKYNVKFEDTIDFLDKIILPYTIILPINSHDYEKAKGIMISKTLKPSDAFHVAVMINNSIKKIVSEDSDFDK
INGIERIWVK

Nucleotide

• Length: 273 bp

>T113465 NZ_CP033240:c2345533-2345261 [Saccharolobus solfataricus]
ATGAGGTTATTTACATTATCAAAAAAGAAGTATAATGTAAAATTTGAGGATACAATTGATTTCTTAGATAAGATAATACT
TCCTTATACAATTATACTGCCAATCAACTCTCATGATTATGAAAAAGCAAAGGGGATAATGATAAGTAAGACTTTAAAAC
CGTCTGATGCTTTCCACGTAGCTGTAATGATAAATAATTCAATAAAAAAGATAGTAAGTGAGGATTCAGATTTCGATAAA
ATTAATGGAATAGAAAGGATTTGGGTAAAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9842.51 Da
• Isoelectric Point: 5.2456

>AT113465 WP_009990356.1 NZ_CP033240:c2345908-2345648 [Saccharolobus solfataricus]
MTLKIEVGKKGYIIIPKTVRDLLEIKEGDILILRVEDGKIVLEPERKVSFDDLKKKMEEHSAKISYANKAKLGDLINVSL
EEEFDN

Nucleotide

• Length: 261 bp

>AT113465 NZ_CP033240:c2345908-2345648 [Saccharolobus solfataricus]
TTGACCCTAAAGATTGAAGTTGGAAAGAAAGGATATATAATAATCCCTAAGACTGTTAGGGATCTTCTTGAAATAAAGGA
GGGAGATATATTAATCCTAAGAGTCGAAGATGGTAAAATTGTTCTTGAGCCTGAAAGAAAGGTAAGTTTTGATGACTTAA
AAAAGAAGATGGAGGAACATAGTGCTAAAATCTCATATGCAAATAAGGCTAAACTTGGAGATTTAATTAACGTTAGTTTA
GAGGAGGAATTCGATAATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E3K976