Detailed information of TA system TA113442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1985646..1986252
• Replicon: chromosome
• Accession: NZ_CP033237
• Organism: Saccharolobus solfataricus strain SARC-I

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q97YS3
• Locus tag: SULI_RS11305
• Protein ID: WP_009993017.1
• Coordinates: 1985860..1986252 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E3MAZ1
• Locus tag: SULI_RS11300
• Protein ID: WP_014511634.1
• Coordinates: 1985646..1985870 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SULI_RS11275 (SULI_11280)	1980794..1981924	+	1131	WP_009992604.1	aromatic/alkene monooxygenase hydroxylase subunit beta	-
SULI_RS11280 (SULI_11285)	1981921..1982382	+	462	WP_010923265.1	metal-sulfur cluster assembly factor	-
SULI_RS11285 (SULI_11290)	1982425..1983340	-	916	Protein_2185	IS110 family transposase	-
SULI_RS11290 (SULI_11295)	1983385..1984368	+	984	WP_010923242.1	IS630-like element ISC1048 family transposase	-
SULI_RS11295 (SULI_11300)	1984367..1985336	+	970	Protein_2187	IS110 family transposase	-
SULI_RS11300 (SULI_11305)	1985646..1985870	+	225	WP_014511634.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
SULI_RS11305 (SULI_11310)	1985860..1986252	+	393	WP_009993017.1	type II toxin-antitoxin system VapC family toxin	Toxin
SULI_RS11310 (SULI_11315)	1986695..1987173	-	479	Protein_2190	ISNCY family transposase	-
SULI_RS11315 (SULI_11320)	1987291..1988343	+	1053	WP_010923271.1	ISH3 family transposase	-
SULI_RS11320 (SULI_11325)	1988531..1989118	-	588	Protein_2192	ISNCY family transposase	-
SULI_RS11325 (SULI_11330)	1989552..1989893	-	342	WP_009990179.1	nucleotidyltransferase domain-containing protein	-
SULI_RS11330 (SULI_11335)	1989884..1990231	-	348	WP_014511635.1	HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1977369..1989118	11749
	-	inside	IScluster/Tn	-	-	1976524..1989118	12594

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (8-124)

Antitoxin

• Post-segregation antitoxin CcdA (5-49)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 15117.81 Da
• Isoelectric Point: 9.3474

>T113442 WP_009993017.1 NZ_CP033237:1985860-1986252 [Saccharolobus solfataricus]
MKDKEFLLDASALYSLLDYVDKVDVKKIHVLTLTFYEVGNVIWKEYYIHKKVKDPITLSRLFYKLMRKFNVIEDSPLEGV
MRIAIERGLTYYNASYAYVAESLGLILVSNDKELIRKANAISLKDLIKSM

Nucleotide

• Length: 393 bp

>T113442 NZ_CP033237:1985860-1986252 [Saccharolobus solfataricus]
ATGAAAGATAAGGAATTCTTGCTCGATGCCTCAGCTTTGTATTCACTTTTAGATTACGTGGATAAGGTAGATGTTAAGAA
AATTCATGTACTTACCCTAACTTTTTATGAGGTAGGTAATGTCATATGGAAGGAGTATTATATACATAAAAAGGTTAAAG
ATCCTATAACCCTTTCGAGGCTTTTCTATAAATTAATGCGAAAATTTAACGTAATAGAGGATTCGCCTCTTGAGGGGGTA
ATGAGAATTGCCATAGAAAGGGGTTTAACTTACTATAACGCATCTTATGCGTATGTTGCTGAATCTTTAGGGCTTATCCT
AGTGTCTAACGATAAAGAGTTGATAAGAAAGGCTAACGCTATTTCGTTAAAGGATTTGATAAAAAGTATGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8792.11 Da
• Isoelectric Point: 4.8365

>AT113442 WP_014511634.1 NZ_CP033237:1985646-1985870 [Saccharolobus solfataricus]
MSDVISVRVKKELKKKAEELGINVREVVEKALEEAIREKEKEELKDMTMKIKELMRDVSEDDWVSTVRESRDER

Nucleotide

• Length: 225 bp

>AT113442 NZ_CP033237:1985646-1985870 [Saccharolobus solfataricus]
ATGTCAGACGTAATAAGCGTAAGAGTGAAGAAAGAGCTAAAGAAGAAGGCAGAGGAGTTAGGAATTAACGTTAGGGAAGT
TGTAGAAAAAGCTTTAGAGGAAGCCATAAGGGAGAAGGAGAAGGAAGAGCTTAAGGATATGACTATGAAAATTAAGGAAC
TAATGAGGGACGTAAGTGAAGATGATTGGGTAAGTACTGTTAGGGAGAGTAGGGATGAAAGATAA

Structures

Toxin

• AlphaFold DB: A0A0E3GVD1

Antitoxin

• AlphaFold DB: A0A0E3MAZ1