Detailed information of TA system TA113422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1094192..1094816
• Replicon: chromosome
• Accession: NZ_CP033230
• Organism: Sphingobium yanoikuyae strain SJTF8

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: EBF16_RS09190
• Protein ID: WP_037474511.1
• Coordinates: 1094508..1094816 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: EBF16_RS09185
• Protein ID: WP_197421464.1
• Coordinates: 1094192..1094476 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBF16_RS09170	1089509..1090132	+	624	WP_099232135.1	TetR/AcrR family transcriptional regulator	-
EBF16_RS09175	1090209..1092560	-	2352	WP_099232136.1	hypothetical protein	-
EBF16_RS09180	1092714..1094144	+	1431	WP_037474520.1	aldehyde dehydrogenase family protein	-
EBF16_RS09185	1094192..1094476	-	285	WP_197421464.1	BrnA antitoxin family protein	Antitoxin
EBF16_RS09190	1094508..1094816	-	309	WP_037474511.1	BrnT family toxin	Toxin
EBF16_RS09195	1094844..1095431	-	588	WP_025290601.1	recombinase family protein	-
EBF16_RS09200	1095861..1096262	-	402	WP_004207001.1	DUF2147 domain-containing protein	-
EBF16_RS09205	1096275..1097867	-	1593	WP_025290602.1	GMC family oxidoreductase N-terminal domain-containing protein	-
EBF16_RS09210	1097872..1098405	-	534	WP_081912838.1	SMI1/KNR4 family protein	-
EBF16_RS09215	1098517..1099716	-	1200	Protein_1023	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	ugd / gmd / fcl / fcl / gmd / ugd	1001018..1446032	445014
	-	flank	IS/Tn	-	-	1094844..1095431	587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (8-93)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (1-82)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11845.32 Da
• Isoelectric Point: 6.6474

>T113422 WP_037474511.1 NZ_CP033230:c1094816-1094508 [Sphingobium yanoikuyae]
MPSMRFSWHPAKAESNLKKHGVSFAIATRAFTDPFALSDQDRIEGGERRWQTLGLVEGHLLLLVAHTVVEQDDDGEYVEI
IHIISARKADRTERRRYEEERR

Nucleotide

• Length: 309 bp

>T113422 NZ_CP033230:c1094816-1094508 [Sphingobium yanoikuyae]
ATGCCATCCATGCGCTTCTCCTGGCATCCGGCCAAAGCCGAGAGCAATCTGAAGAAGCACGGCGTCAGCTTTGCGATCGC
TACTCGTGCGTTCACAGATCCTTTCGCGCTGAGCGATCAGGATCGGATCGAAGGTGGCGAGCGACGATGGCAAACCTTGG
GCCTCGTGGAAGGGCATCTGCTCCTCCTCGTGGCCCACACTGTCGTCGAGCAAGACGATGACGGCGAGTATGTGGAGATC
ATTCATATCATTTCGGCTCGAAAGGCCGATCGAACGGAAAGGCGACGTTATGAAGAAGAAAGACGCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10764.24 Da
• Isoelectric Point: 9.9870

>AT113422 WP_197421464.1 NZ_CP033230:c1094476-1094192 [Sphingobium yanoikuyae]
MPPLTEAQQAELDALQAMPDSGIDYSDSPALTEDFWRNGQRGRFYKPIKQQVTARLDADVLAWLKSQGKGYQARMNAILR
REMLATARRQEKPR

Nucleotide

• Length: 285 bp

>AT113422 NZ_CP033230:c1094476-1094192 [Sphingobium yanoikuyae]
CTGCCGCCGTTGACCGAAGCGCAACAGGCCGAACTGGATGCCCTGCAGGCGATGCCGGATAGCGGGATCGACTATAGCGA
CTCTCCGGCGCTGACAGAGGATTTCTGGCGGAACGGGCAGCGTGGGCGCTTCTATAAGCCCATCAAGCAGCAGGTGACGG
CACGCCTTGATGCTGATGTGCTCGCCTGGCTCAAATCGCAGGGTAAGGGCTACCAAGCCCGCATGAACGCCATACTTCGC
CGTGAGATGCTGGCGACCGCTAGAAGGCAGGAAAAGCCACGTTAA