113421

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/Tad-HTH_37
Location 288089..288786 Replicon plasmid pF1
Accession NZ_CP033227
Organism Sphingobium yanoikuyae strain SJTF8

Toxin (Protein)


Gene name tad Uniprot ID T0J3S2
Locus tag EBF16_RS01460 Protein ID WP_013041565.1
Coordinates 288089..288463 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID T0G937
Locus tag EBF16_RS01465 Protein ID WP_006964370.1
Coordinates 288460..288786 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EBF16_RS01425 283171..284070 + 900 WP_066522347.1 pirin family protein -
EBF16_RS01430 284135..284437 - 303 WP_066522351.1 hypothetical protein -
EBF16_RS01435 284873..285586 + 714 Protein_278 IS6 family transposase -
EBF16_RS01440 285628..286851 - 1224 WP_014082638.1 IS256-like element ISSpwi2 family transposase -
EBF16_RS01445 287103..287249 + 147 Protein_280 oxidoreductase -
EBF16_RS01450 287254..287394 + 141 WP_122129116.1 hypothetical protein -
EBF16_RS01460 288089..288463 + 375 WP_013041565.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EBF16_RS01465 288460..288786 + 327 WP_006964370.1 helix-turn-helix domain-containing protein Antitoxin
EBF16_RS01470 288786..289028 + 243 WP_013041566.1 hypothetical protein -
EBF16_RS01475 289171..289758 - 588 Protein_285 Tn3 family transposase -
EBF16_RS01485 290155..291021 + 867 WP_122129117.1 recombinase family protein -
EBF16_RS01490 290912..292462 + 1551 WP_080604537.1 catalase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul4 - 1..505328 505328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-108)

Antitoxin

HigA2-like, helix-turn-helix domain

(15-94)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.67 Da        Isoelectric Point: 9.4885

>T113421 WP_013041565.1 NZ_CP033227:288089-288463 [Sphingobium yanoikuyae]
MDTERPVRWVASSKRDFREFPDDVQDVMGYALHLAQQGGQHASTKPLKGFGGAGVVEIIDDHQGDTFRTVYTVKFAEAVY
VLHAFQKKSKQGKATPQADMDLIRTRLKSAEEHHRQHQTPRGTA

Download         Length: 375 bp

>T113421 NZ_CP033227:288089-288463 [Sphingobium yanoikuyae]
ATGGATACGGAACGGCCGGTTCGATGGGTGGCATCGTCAAAGCGCGATTTTCGGGAGTTCCCGGACGATGTGCAGGACGT
AATGGGCTATGCCCTGCACCTTGCGCAGCAAGGCGGGCAGCACGCCAGCACTAAGCCGTTGAAGGGCTTTGGCGGCGCTG
GCGTGGTCGAGATCATAGACGACCACCAAGGCGATACGTTCCGCACGGTCTATACGGTCAAATTCGCGGAGGCGGTCTAT
GTCCTGCACGCCTTCCAGAAGAAATCCAAGCAGGGCAAGGCCACGCCGCAGGCCGATATGGATTTGATCCGAACGCGGCT
GAAATCCGCCGAGGAGCATCACCGGCAACACCAGACCCCGCGAGGCACAGCATGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11792.57 Da        Isoelectric Point: 7.1681

>AT113421 WP_006964370.1 NZ_CP033227:288460-288786 [Sphingobium yanoikuyae]
MSAQDEIIEEGSGNVFADLGFADPDTHLLKAQLVTRVAEAMQERKLTQIAAAKVTGSTQPELSRILRGQFRSVSVERLLA
MLTRLGCKVDIVVRPQGRTTESAVIHFA

Download         Length: 327 bp

>AT113421 NZ_CP033227:288460-288786 [Sphingobium yanoikuyae]
ATGAGCGCACAGGACGAAATCATTGAGGAAGGCAGCGGCAATGTCTTTGCCGATCTAGGCTTTGCCGACCCCGACACCCA
CCTTTTGAAAGCCCAGCTCGTTACCCGTGTGGCCGAGGCCATGCAGGAGCGGAAGTTGACGCAGATCGCCGCCGCCAAGG
TTACGGGATCTACCCAACCGGAATTGTCGCGCATCCTGCGAGGCCAGTTCCGCAGCGTCTCGGTTGAGCGGCTTCTAGCC
ATGCTCACCCGGCTTGGCTGCAAGGTCGATATTGTCGTGCGCCCGCAGGGGCGGACCACTGAATCGGCCGTGATCCACTT
CGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J7XHG4

Antitoxin

Source ID Structure
AlphaFold DB A0A0J8A803

References