Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relB-parE/ParE-RHH
Location 259859..260418 Replicon plasmid pF1
Accession NZ_CP033227
Organism Sphingobium yanoikuyae strain SJTF8

Toxin (Protein)


Gene name parE Uniprot ID A0A177JTL3
Locus tag EBF16_RS01305 Protein ID WP_063976427.1
Coordinates 259859..260152 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A177JTD8
Locus tag EBF16_RS01310 Protein ID WP_063976426.1
Coordinates 260140..260418 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EBF16_RS01275 255488..256945 + 1458 WP_122129195.1 aldehyde dehydrogenase family protein -
EBF16_RS01280 257070..257555 - 486 WP_063976431.1 hypothetical protein -
EBF16_RS01285 257606..257848 - 243 WP_063976430.1 hypothetical protein -
EBF16_RS01290 257925..258623 + 699 WP_081260992.1 TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator -
EBF16_RS01295 259176..259433 + 258 WP_063976429.1 hypothetical protein -
EBF16_RS01300 259430..259756 + 327 WP_063976428.1 type II toxin-antitoxin system PemK/MazF family toxin -
EBF16_RS01305 259859..260152 - 294 WP_063976427.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EBF16_RS01310 260140..260418 - 279 WP_063976426.1 CopG family ribbon-helix-helix protein Antitoxin
EBF16_RS01315 260968..261732 - 765 WP_015449415.1 IS6-like element IS6100 family transposase -
EBF16_RS01325 261816..262145 + 330 WP_004213249.1 heavy-metal-associated domain-containing protein -
EBF16_RS01330 262183..263601 + 1419 WP_206379267.1 mercury(II) reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul4 - 1..505328 505328
- flank IS/Tn - - 260968..261732 764


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-94)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-42)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10767.35 Da        Isoelectric Point: 7.3163

>T113420 WP_063976427.1 NZ_CP033227:c260152-259859 [Sphingobium yanoikuyae]
MPRLIWTPSALADVERLHNWLKPTDADAARRAVAAIRAGVTVLAQSPHVGRPMEEMDPEYREKLIAFGNSGYVALYRLDG
DVVAILAVRHQKESGYP

Download         Length: 294 bp

>T113420 NZ_CP033227:c260152-259859 [Sphingobium yanoikuyae]
TTGCCACGTCTGATCTGGACGCCTAGCGCATTGGCTGACGTGGAGCGGCTTCATAATTGGTTGAAGCCCACGGATGCCGA
TGCAGCGCGGCGCGCGGTAGCAGCGATCAGGGCAGGGGTGACGGTTCTCGCGCAATCGCCGCACGTCGGCCGCCCGATGG
AAGAGATGGACCCCGAATATCGGGAGAAACTAATTGCCTTTGGGAATAGCGGCTATGTTGCCCTGTATCGCCTCGACGGC
GACGTGGTCGCAATCCTAGCCGTTCGCCACCAGAAGGAATCTGGCTATCCATAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10741.03 Da        Isoelectric Point: 5.2308

>AT113420 WP_063976426.1 NZ_CP033227:c260418-260140 [Sphingobium yanoikuyae]
MAAATSIKLDDTLKGRVQHLAEARRRSSHWIMREAIAQYVEREEKREAFRRDAIEAWEEYQRTGMHLTFEEADAWLAKLE
AGEDAELPNCHV

Download         Length: 279 bp

>AT113420 NZ_CP033227:c260418-260140 [Sphingobium yanoikuyae]
ATGGCAGCGGCAACATCTATAAAGCTCGATGATACCCTGAAGGGGCGCGTGCAGCACTTGGCCGAAGCTCGTCGGCGCAG
TTCACATTGGATCATGCGCGAGGCTATCGCACAGTATGTCGAACGAGAAGAAAAGCGTGAGGCGTTCAGGCGAGATGCGA
TTGAGGCATGGGAAGAATATCAGCGAACAGGGATGCATCTGACGTTTGAGGAGGCAGATGCCTGGCTGGCGAAGCTGGAA
GCGGGCGAGGATGCGGAACTGCCTAATTGCCACGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A177JTL3

Antitoxin

Source ID Structure
AlphaFold DB A0A177JTD8

References