Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | pemIK/PRK09812-MazE |
Location | 259176..259756 | Replicon | plasmid pF1 |
Accession | NZ_CP033227 | ||
Organism | Sphingobium yanoikuyae strain SJTF8 |
Toxin (Protein)
Gene name | pemK | Uniprot ID | A0A177JUB3 |
Locus tag | EBF16_RS01300 | Protein ID | WP_063976428.1 |
Coordinates | 259430..259756 (+) | Length | 109 a.a. |
Antitoxin (Protein)
Gene name | pemI | Uniprot ID | A0A177JVZ4 |
Locus tag | EBF16_RS01295 | Protein ID | WP_063976429.1 |
Coordinates | 259176..259433 (+) | Length | 86 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
EBF16_RS01270 | 254265..255389 | + | 1125 | WP_122129113.1 | NAD(P)-dependent alcohol dehydrogenase | - |
EBF16_RS01275 | 255488..256945 | + | 1458 | WP_122129195.1 | aldehyde dehydrogenase family protein | - |
EBF16_RS01280 | 257070..257555 | - | 486 | WP_063976431.1 | hypothetical protein | - |
EBF16_RS01285 | 257606..257848 | - | 243 | WP_063976430.1 | hypothetical protein | - |
EBF16_RS01290 | 257925..258623 | + | 699 | WP_081260992.1 | TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator | - |
EBF16_RS01295 | 259176..259433 | + | 258 | WP_063976429.1 | hypothetical protein | Antitoxin |
EBF16_RS01300 | 259430..259756 | + | 327 | WP_063976428.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
EBF16_RS01305 | 259859..260152 | - | 294 | WP_063976427.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
EBF16_RS01310 | 260140..260418 | - | 279 | WP_063976426.1 | CopG family ribbon-helix-helix protein | - |
EBF16_RS01315 | 260968..261732 | - | 765 | WP_015449415.1 | IS6-like element IS6100 family transposase | - |
EBF16_RS01325 | 261816..262145 | + | 330 | WP_004213249.1 | heavy-metal-associated domain-containing protein | - |
EBF16_RS01330 | 262183..263601 | + | 1419 | WP_206379267.1 | mercury(II) reductase | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Mobilizable plasmid | sul4 | - | 1..505328 | 505328 | |
- | flank | IS/Tn | - | - | 260968..261732 | 764 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 109 a.a. Molecular weight: 11899.77 Da Isoelectric Point: 10.0037
>T113419 WP_063976428.1 NZ_CP033227:259430-259756 [Sphingobium yanoikuyae]
VKRGDVYMVDLEPTHGREQRGHRPVVIVSPDDFNRATGLPLIVPITNGGDFARRIGFAVPLSGTKTTGVVRCDQPRVLDL
KIRNGRKVETLPQAIMDEVLARTATLLE
VKRGDVYMVDLEPTHGREQRGHRPVVIVSPDDFNRATGLPLIVPITNGGDFARRIGFAVPLSGTKTTGVVRCDQPRVLDL
KIRNGRKVETLPQAIMDEVLARTATLLE
Download Length: 327 bp
>T113419 NZ_CP033227:259430-259756 [Sphingobium yanoikuyae]
GTGAAGCGTGGCGACGTTTACATGGTCGATCTCGAACCGACGCACGGGCGGGAACAGCGCGGCCATCGTCCCGTGGTCAT
TGTCTCGCCCGATGATTTCAACCGCGCCACGGGCTTGCCACTGATCGTCCCAATTACCAATGGCGGCGATTTTGCCCGCC
GTATCGGATTCGCGGTGCCGCTTTCCGGCACGAAAACAACGGGCGTCGTGCGTTGCGATCAACCTCGCGTTCTTGACCTG
AAAATCCGCAACGGCCGCAAGGTGGAAACCTTGCCGCAAGCGATCATGGATGAAGTCTTGGCCCGGACGGCAACGCTTTT
GGAATGA
GTGAAGCGTGGCGACGTTTACATGGTCGATCTCGAACCGACGCACGGGCGGGAACAGCGCGGCCATCGTCCCGTGGTCAT
TGTCTCGCCCGATGATTTCAACCGCGCCACGGGCTTGCCACTGATCGTCCCAATTACCAATGGCGGCGATTTTGCCCGCC
GTATCGGATTCGCGGTGCCGCTTTCCGGCACGAAAACAACGGGCGTCGTGCGTTGCGATCAACCTCGCGTTCTTGACCTG
AAAATCCGCAACGGCCGCAAGGTGGAAACCTTGCCGCAAGCGATCATGGATGAAGTCTTGGCCCGGACGGCAACGCTTTT
GGAATGA
Antitoxin
Download Length: 86 a.a. Molecular weight: 8903.25 Da Isoelectric Point: 4.9731
>AT113419 WP_063976429.1 NZ_CP033227:259176-259433 [Sphingobium yanoikuyae]
MHTTNLRKVGGSVMLAVPPALLDVLHLAAGAKVGLAVDNGKLVVEPGARPRYTMAELLGASDYSQPQPPEEREWVDASAV
GGELL
MHTTNLRKVGGSVMLAVPPALLDVLHLAAGAKVGLAVDNGKLVVEPGARPRYTMAELLGASDYSQPQPPEEREWVDASAV
GGELL
Download Length: 258 bp
>AT113419 NZ_CP033227:259176-259433 [Sphingobium yanoikuyae]
ATGCACACAACTAATCTTAGGAAGGTCGGCGGCTCCGTCATGCTCGCCGTACCCCCCGCGCTACTGGACGTTCTGCACCT
TGCGGCGGGCGCAAAGGTCGGGCTTGCCGTCGATAATGGCAAGCTGGTGGTGGAGCCTGGCGCCCGTCCCCGCTACACGA
TGGCCGAATTGCTGGGCGCGTCCGACTATTCGCAGCCGCAGCCGCCAGAGGAGCGGGAATGGGTTGATGCTTCGGCTGTC
GGTGGCGAGCTTCTGTGA
ATGCACACAACTAATCTTAGGAAGGTCGGCGGCTCCGTCATGCTCGCCGTACCCCCCGCGCTACTGGACGTTCTGCACCT
TGCGGCGGGCGCAAAGGTCGGGCTTGCCGTCGATAATGGCAAGCTGGTGGTGGAGCCTGGCGCCCGTCCCCGCTACACGA
TGGCCGAATTGCTGGGCGCGTCCGACTATTCGCAGCCGCAGCCGCCAGAGGAGCGGGAATGGGTTGATGCTTCGGCTGTC
GGTGGCGAGCTTCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A177JUB3 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A177JVZ4 |