Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 48361..48968 Replicon plasmid pRBWH1.2
Accession NZ_CP033208
Organism Enterococcus faecium strain RBWH1

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag EBB55_RS15800 Protein ID WP_002321032.1
Coordinates 48618..48968 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag EBB55_RS15795 Protein ID WP_002287514.1
Coordinates 48361..48624 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EBB55_RS15775 43913..44740 - 828 WP_002321275.1 hypothetical protein -
EBB55_RS15780 44836..45492 - 657 WP_101706206.1 class C sortase -
EBB55_RS15785 45505..47268 - 1764 Protein_46 isopeptide-forming domain-containing fimbrial protein -
EBB55_RS15790 47325..48005 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EBB55_RS16495 48043..48207 - 165 WP_164450253.1 hypothetical protein -
EBB55_RS15795 48361..48624 + 264 WP_002287514.1 hypothetical protein Antitoxin
EBB55_RS15800 48618..48968 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EBB55_RS15805 48992..49606 - 615 WP_002318230.1 recombinase family protein -
EBB55_RS15810 49920..50342 + 423 WP_002288787.1 hypothetical protein -
EBB55_RS15815 50352..50555 + 204 WP_002299573.1 hypothetical protein -
EBB55_RS15820 50766..51350 + 585 WP_002299575.1 recombinase family protein -
EBB55_RS15825 51721..53046 + 1326 WP_002303115.1 Y-family DNA polymerase -
EBB55_RS15830 53039..53389 + 351 WP_002303114.1 hypothetical protein -
EBB55_RS15835 53386..53565 + 180 WP_001021554.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 47325..48011 686
- inside Non-Mobilizable plasmid - - 1..55808 55808


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T113406 WP_002321032.1 NZ_CP033208:48618-48968 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T113406 NZ_CP033208:48618-48968 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT113406 WP_002287514.1 NZ_CP033208:48361-48624 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT113406 NZ_CP033208:48361-48624 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References