Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 515414..515985 Replicon chromosome
Accession NZ_CP033206
Organism Enterococcus faecium strain RBWH1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag EBB55_RS02665 Protein ID WP_002286801.1
Coordinates 515644..515985 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag EBB55_RS02660 Protein ID WP_002323011.1
Coordinates 515414..515644 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EBB55_RS02610 510852..511478 + 627 WP_002286816.1 cysteine hydrolase -
EBB55_RS02615 511661..512242 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
EBB55_RS02635 512974..513321 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
EBB55_RS02640 513297..513536 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
EBB55_RS02645 513741..514079 - 339 WP_002286804.1 hypothetical protein -
EBB55_RS02655 514452..515360 + 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
EBB55_RS02660 515414..515644 + 231 WP_002323011.1 hypothetical protein Antitoxin
EBB55_RS02665 515644..515985 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EBB55_RS02680 518204..518389 + 186 WP_002304207.1 hypothetical protein -
EBB55_RS02685 518894..519088 + 195 WP_002297028.1 hypothetical protein -
EBB55_RS02690 519246..519528 + 283 Protein_493 transposase -
EBB55_RS02700 519772..520602 - 831 WP_002286789.1 manganese catalase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element VanHBX - 441251..692462 251211
- inside IScluster/Tn - - 514452..518019 3567


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T113405 WP_002286801.1 NZ_CP033206:515644-515985 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T113405 NZ_CP033206:515644-515985 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT113405 WP_002323011.1 NZ_CP033206:515414-515644 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT113405 NZ_CP033206:515414-515644 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References