Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518663..519299 Replicon chromosome
Accession NZ_CP033205
Organism Bacillus subtilis strain MBI 600

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag DA787_RS02675 Protein ID WP_003156187.1
Coordinates 518949..519299 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag DA787_RS02670 Protein ID WP_003225183.1
Coordinates 518663..518944 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DA787_RS02650 515022..515621 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
DA787_RS02655 515716..516081 + 366 WP_003234281.1 holo-ACP synthase -
DA787_RS02660 516247..517263 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
DA787_RS02665 517378..518547 + 1170 WP_003234284.1 alanine racemase -
DA787_RS02670 518663..518944 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
DA787_RS02675 518949..519299 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DA787_RS02680 519414..520238 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
DA787_RS02685 520243..520608 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
DA787_RS02690 520612..521013 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
DA787_RS02695 521025..522032 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
DA787_RS02700 522094..522423 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
DA787_RS02705 522420..522902 + 483 WP_003234299.1 anti-sigma B factor RsbW -
DA787_RS02710 522868..523656 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
DA787_RS02715 523656..524255 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T113395 WP_003156187.1 NZ_CP033205:518949-519299 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T113395 NZ_CP033205:518949-519299 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT113395 WP_003225183.1 NZ_CP033205:518663-518944 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT113395 NZ_CP033205:518663-518944 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References