Detailed information of TA system TA113394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1521650..1522239
• Replicon: chromosome
• Accession: NZ_CP033204
• Organism: Sphingopyxis sp. YF1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EAO27_RS07390
• Protein ID: WP_242778964.1
• Coordinates: 1521871..1522239 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EAO27_RS07385
• Protein ID: WP_242778961.1
• Coordinates: 1521650..1521874 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAO27_RS07355 (EAO27_07235)	1516676..1517542	+	867	WP_242778943.1	HprK-related kinase A	-
EAO27_RS07360 (EAO27_07240)	1517539..1518552	+	1014	WP_242778946.1	nucleotidyltransferase family protein	-
EAO27_RS07365 (EAO27_07245)	1518593..1519258	-	666	WP_242778949.1	HAD-IA family hydrolase	-
EAO27_RS07370 (EAO27_07250)	1519420..1520766	+	1347	WP_242778952.1	bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU	-
EAO27_RS07375 (EAO27_07255)	1520769..1521308	+	540	WP_242778955.1	GNAT family N-acetyltransferase	-
EAO27_RS07380 (EAO27_07260)	1521305..1521619	+	315	WP_242778958.1	hypothetical protein	-
EAO27_RS07385 (EAO27_07265)	1521650..1521874	+	225	WP_242778961.1	ribbon-helix-helix domain-containing protein	Antitoxin
EAO27_RS07390 (EAO27_07270)	1521871..1522239	+	369	WP_242778964.1	PIN domain-containing protein	Toxin
EAO27_RS07395 (EAO27_07275)	1522254..1524077	+	1824	WP_242778968.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
EAO27_RS07400	1524329..1524760	+	432	WP_278190158.1	SMI1/KNR4 family protein	-
EAO27_RS07405 (EAO27_07285)	1524919..1525170	+	252	WP_242778974.1	type II toxin-antitoxin system ParD family antitoxin	-
EAO27_RS07410 (EAO27_07290)	1525167..1525478	+	312	WP_242778977.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EAO27_RS07415 (EAO27_07295)	1525498..1525686	-	189	WP_242778980.1	hypothetical protein	-
EAO27_RS07420 (EAO27_07300)	1525756..1526415	+	660	WP_242778983.1	HAD family phosphatase	-
EAO27_RS07425 (EAO27_07305)	1526437..1526709	+	273	WP_242778986.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-105)

Antitoxin

• Ribbon-helix-helix protein, CopG (7-39)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13044.17 Da
• Isoelectric Point: 8.5316

>T113394 WP_242778964.1 NZ_CP033204:1521871-1522239 [Sphingopyxis sp. YF1]
MIDAQFDSDILIDALNGVEAARSEIRRAGRKSVSRISWTEVMTAADPASVKAVEAFLGCFAVEEVGDAVARRAAALRAER
KGLTLCDALVLATAQISGRILVTRNIKVFPAAMPGIRVPYTL

Nucleotide

• Length: 369 bp

>T113394 NZ_CP033204:1521871-1522239 [Sphingopyxis sp. YF1]
ATGATCGACGCGCAGTTCGACAGCGACATCCTCATCGACGCCTTGAACGGCGTCGAGGCGGCGCGCAGCGAAATCCGACG
CGCGGGGCGCAAGAGCGTCAGCCGGATCAGCTGGACCGAGGTGATGACCGCCGCCGACCCCGCGTCGGTGAAGGCGGTCG
AGGCCTTCCTCGGCTGCTTTGCGGTCGAGGAGGTCGGCGATGCCGTCGCGCGCCGCGCCGCCGCGCTGCGTGCCGAGCGC
AAGGGGCTGACGCTCTGCGACGCGCTGGTGCTCGCGACGGCGCAGATTTCGGGGCGCATTCTCGTTACACGCAATATCAA
GGTGTTTCCGGCAGCGATGCCGGGTATTCGCGTTCCCTACACGCTCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8275.22 Da
• Isoelectric Point: 6.3192

>AT113394 WP_242778961.1 NZ_CP033204:1521650-1521874 [Sphingopyxis sp. YF1]
VRFLADIPDSDVEWLDALAAEQGVSRAELVRRAVASYRAEASGGAIDNAFGIWRDRADIGDGLRYQRRLRGKRE

Nucleotide

• Length: 225 bp

>AT113394 NZ_CP033204:1521650-1521874 [Sphingopyxis sp. YF1]
GTGCGCTTCCTTGCCGACATCCCCGACTCCGACGTCGAATGGCTTGACGCGCTCGCGGCCGAGCAGGGCGTTTCGCGCGC
CGAACTCGTGCGCCGCGCGGTCGCATCCTATCGCGCCGAAGCCTCGGGCGGTGCGATCGACAATGCCTTCGGCATCTGGC
GTGACCGCGCCGACATCGGCGATGGCCTCAGATATCAGCGCCGCCTGCGGGGCAAGCGCGAATGA