Detailed information of TA system TA113387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3434422..3435058
• Replicon: chromosome
• Accession: NZ_CP033198
• Organism: Bacillus paralicheniformis strain FA6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M5P3Q9
• Locus tag: D2B33_RS17340
• Protein ID: WP_003179128.1
• Coordinates: 3434422..3434772 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M5PDU2
• Locus tag: D2B33_RS17345
• Protein ID: WP_006638778.1
• Coordinates: 3434777..3435058 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2B33_RS17300	3429466..3430065	-	600	WP_020450265.1	SpoIIE family protein phosphatase	-
D2B33_RS17305	3430062..3430853	-	792	WP_023857858.1	RNA polymerase sigma factor SigB	-
D2B33_RS17310	3430819..3431301	-	483	WP_020450263.1	anti-sigma B factor RsbW	-
D2B33_RS17315	3431301..3431627	-	327	WP_020450262.1	anti-sigma factor antagonist	-
D2B33_RS17320	3431686..3432693	-	1008	WP_020450261.1	PP2C family protein-serine/threonine phosphatase	-
D2B33_RS17325	3432704..3433105	-	402	WP_020450260.1	anti-sigma regulatory factor	-
D2B33_RS17330	3433108..3433473	-	366	WP_020450259.1	STAS domain-containing protein	-
D2B33_RS17335	3433477..3434304	-	828	WP_020450258.1	RsbT co-antagonist protein RsbRA	-
D2B33_RS17340	3434422..3434772	-	351	WP_003179128.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
D2B33_RS17345	3434777..3435058	-	282	WP_006638778.1	hypothetical protein	Antitoxin
D2B33_RS17350	3435170..3436339	-	1170	WP_041817156.1	alanine racemase	-
D2B33_RS17355	3436565..3437524	-	960	WP_165395380.1	outer membrane lipoprotein carrier protein LolA	-
D2B33_RS17360	3437868..3438467	+	600	WP_020450255.1	rhomboid family intramembrane serine protease	-
D2B33_RS17365	3438464..3439948	-	1485	WP_041817155.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12990.03 Da
• Isoelectric Point: 5.7234

>T113387 WP_003179128.1 NZ_CP033198:c3434772-3434422 [Bacillus paralicheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Nucleotide

• Length: 351 bp

>T113387 NZ_CP033198:c3434772-3434422 [Bacillus paralicheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10649.03 Da
• Isoelectric Point: 4.6920

>AT113387 WP_006638778.1 NZ_CP033198:c3435058-3434777 [Bacillus paralicheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT113387 NZ_CP033198:c3435058-3434777 [Bacillus paralicheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATTAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A6I7FHI4

Antitoxin

• AlphaFold DB: M5PDU2