Detailed information of TA system TA113386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 2587103..2588022
• Replicon: chromosome
• Accession: NZ_CP033198
• Organism: Bacillus paralicheniformis strain FA6

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: D2B33_RS13075
• Protein ID: WP_020451047.1
• Coordinates: 2587103..2587849 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: -
• Locus tag: D2B33_RS13080
• Protein ID: WP_020451046.1
• Coordinates: 2587849..2588022 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2B33_RS13055	2582495..2583139	-	645	WP_020451052.1	YesL family protein	-
D2B33_RS13060	2583276..2585033	-	1758	WP_020451051.1	gamma-glutamyltransferase	-
D2B33_RS13065	2585365..2585982	+	618	WP_020451049.1	DUF47 domain-containing protein	-
D2B33_RS13070	2585995..2586993	+	999	WP_020451048.1	inorganic phosphate transporter	-
D2B33_RS13075	2587103..2587849	+	747	WP_020451047.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
D2B33_RS13080	2587849..2588022	+	174	WP_020451046.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
D2B33_RS13085	2588318..2589589	-	1272	WP_122934918.1	MFS transporter	-
D2B33_RS13090	2589641..2590654	-	1014	WP_020451044.1	zinc-binding alcohol dehydrogenase family protein	-
D2B33_RS13095	2590682..2591530	-	849	WP_020451043.1	SDR family oxidoreductase	-
D2B33_RS13100	2591506..2592603	-	1098	WP_020451042.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29435.89 Da
• Isoelectric Point: 4.6065

>T113386 WP_020451047.1 NZ_CP033198:2587103-2587849 [Bacillus paralicheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKKLTEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLERKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T113386 NZ_CP033198:2587103-2587849 [Bacillus paralicheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTCATTGTTTTGGCGCTGGCTCTGTATGTAGCTGCCGTGTGGCGTTTTGA
AAAAAAGCTCACCGAAAAAACGGTCGCGATCCGAAAAACGTGGTACCTGCTTTATGTTATCGGCGCCGTGATTTATTGGA
CGCACGATCCGCAGTCGATTTTCACAAACCCGCTTCATTACTTGATCGTTGCCGTTTTTTTCACGCTGACAGACGCTTTT
ATTTTCTTAAACGCCTACTTTAAAAAGCTCGGCAGCTCCGAACTCGCGACAGACACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACATATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGGATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
GCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGACTACCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6926.00 Da
• Isoelectric Point: 10.9186

>AT113386 WP_020451046.1 NZ_CP033198:2587849-2588022 [Bacillus paralicheniformis]
MEPAFQQDHESAKKRLAFKMFKRQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT113386 NZ_CP033198:2587849-2588022 [Bacillus paralicheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCATGAAAGCGCAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAGGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA