Detailed information of TA system TA113366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 2255290..2255846
• Replicon: chromosome
• Accession: NZ_CP033192
• Organism: Xanthomonas oryzae pv. oryzae strain NX0260

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: EBA21_RS11065
• Protein ID: WP_041182647.1
• Coordinates: 2255290..2255580 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A854CIX2
• Locus tag: EBA21_RS11070
• Protein ID: WP_011408759.1
• Coordinates: 2255568..2255846 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBA21_RS11015	2251396..2251712	-	317	Protein_2016	ATP-dependent DNA ligase	-
EBA21_RS25025	2251772..2251978	-	207	Protein_2017	hypothetical protein	-
EBA21_RS11025	2251994..2252170	-	177	Protein_2018	DUF3606 domain-containing protein	-
EBA21_RS11030	2252392..2252793	+	402	WP_011259345.1	H-NS histone family protein	-
EBA21_RS11040	2253736..2253737	-	2	WP_094187731.1	IS5 family transposase	-
EBA21_RS11055	2254509..2254688	-	180	WP_015463309.1	hypothetical protein	-
EBA21_RS11060	2254959..2255213	-	255	WP_198912806.1	hypothetical protein	-
EBA21_RS11065	2255290..2255580	-	291	WP_041182647.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EBA21_RS11070	2255568..2255846	-	279	WP_011408759.1	CopG family ribbon-helix-helix protein	Antitoxin
EBA21_RS25305	2255927..2256130	-	204	WP_198912807.1	hypothetical protein	-
EBA21_RS25310	2256042..2256386	-	345	WP_198912817.1	hypothetical protein	-
EBA21_RS11080	2256331..2256531	-	201	WP_027703931.1	ribbon-helix-helix protein, CopG family	-
EBA21_RS11085	2257292..2258476	-	1185	WP_133264606.1	IS256 family transposase	-
EBA21_RS11095	2259057..2260022	+	966	WP_109181970.1	IS1595-like element ISXo5 family transposase	-
EBA21_RS11100	2260124..2260729	+	606	Protein_2030	DNA-3-methyladenine glycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2253209..2260022	6813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10929.51 Da
• Isoelectric Point: 9.5554

>T113366 WP_041182647.1 NZ_CP033192:c2255580-2255290 [Xanthomonas oryzae pv. oryzae]
MPQVIFAPAAIGDMLRLREFLRPKNPDAARRAGEAIRRGVQALGAHPRMGRLIEDLSEQYREWLIDFGDSGYVARYRFDG
DTVTILALRHQKEAGY

Nucleotide

• Length: 291 bp

>T113366 NZ_CP033192:c2255580-2255290 [Xanthomonas oryzae pv. oryzae]
ATGCCACAAGTAATCTTTGCGCCGGCGGCGATCGGGGACATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGA
TGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGTCCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAG
AAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATTTTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGG
GATACCGTCACGATTTTGGCCCTGCGACACCAGAAGGAAGCGGGGTACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10826.11 Da
• Isoelectric Point: 4.9736

>AT113366 WP_011408759.1 NZ_CP033192:c2255846-2255568 [Xanthomonas oryzae pv. oryzae]
MVTATSIKLDDELKGRVQHLAESRRRTPHWIMREAIEQYVEREEKREALNRDTLKAWDEFQATGLHVTAEEVDKWLASWG
TDDELPPPECHK

Nucleotide

• Length: 279 bp

>AT113366 NZ_CP033192:c2255846-2255568 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A854CIX2