Detailed information of TA system TA113338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1749936..1750513
• Replicon: chromosome
• Accession: NZ_CP033184
• Organism: Xanthomonas oryzae pv. oryzae strain CFBP1949

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EBA01_RS08790
• Protein ID: WP_196381723.1
• Coordinates: 1750217..1750513 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A411Y3T7
• Locus tag: EBA01_RS08785
• Protein ID: WP_017154976.1
• Coordinates: 1749936..1750178 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBA01_RS08765	1746099..1746683	-	585	WP_024743447.1	NAD(P)H-dependent oxidoreductase	-
EBA01_RS08770	1746783..1747799	+	1017	WP_024743446.1	LysR family transcriptional regulator	-
EBA01_RS08780	1748351..1749840	+	1490	Protein_1590	site-specific integrase	-
EBA01_RS08785	1749936..1750178	+	243	WP_017154976.1	hypothetical protein	Antitoxin
EBA01_RS08790	1750217..1750513	+	297	WP_196381723.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EBA01_RS08795	1750635..1751000	+	366	Protein_1593	transposase	-
EBA01_RS08800	1751066..1752162	+	1097	Protein_1594	IS3 family transposase	-
EBA01_RS08805	1752181..1752327	+	147	WP_165961480.1	integrase core domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-95)

Antitoxin

• Antitoxin PemI-like (4-76)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10706.15 Da
• Isoelectric Point: 8.0458

>T113338 WP_196381723.1 NZ_CP033184:1750217-1750513 [Xanthomonas oryzae pv. oryzae]
MDDVGGAGHEQRGDRPHLVLSSQGYNDTTSLVVCVPVTTKPKGNPFEVSLNDLNKPSVALTNQVMTADWRSRNAFPRGFA
NKAEMLQVEAKVRALLQL

Nucleotide

• Length: 297 bp

>T113338 NZ_CP033184:1750217-1750513 [Xanthomonas oryzae pv. oryzae]
TTGGATGATGTGGGTGGGGCCGGTCATGAGCAGCGTGGGGACCGGCCACACTTGGTTCTCAGTTCGCAAGGCTACAACGA
CACAACTAGTCTAGTGGTTTGCGTTCCTGTCACAACTAAACCCAAAGGCAATCCTTTTGAAGTAAGCCTTAATGATTTGA
ACAAGCCAAGTGTTGCTTTAACAAATCAGGTCATGACGGCAGATTGGCGCAGTAGAAATGCTTTTCCCCGAGGGTTTGCT
AATAAAGCTGAAATGCTCCAAGTTGAAGCCAAGGTTCGCGCTTTGCTTCAACTTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8786.89 Da
• Isoelectric Point: 4.3753

>AT113338 WP_017154976.1 NZ_CP033184:1749936-1750178 [Xanthomonas oryzae pv. oryzae]
MAVLKMWGNSAAVRIPATYLEESKFSIDEPLVITAEEGRIVIESSKPSYNLADLVAQITPENTHGFAGHSQRVGAEDIEW

Nucleotide

• Length: 243 bp

>AT113338 NZ_CP033184:1749936-1750178 [Xanthomonas oryzae pv. oryzae]
ATGGCTGTTTTGAAGATGTGGGGGAATAGTGCTGCAGTTCGAATCCCGGCGACCTATCTCGAGGAGTCCAAGTTTTCTAT
TGACGAGCCTCTTGTAATCACTGCGGAGGAAGGTCGCATTGTGATCGAGTCGAGTAAGCCGTCTTATAACCTCGCGGACT
TGGTTGCTCAAATCACTCCAGAAAACACGCACGGCTTTGCTGGGCATTCCCAGAGAGTCGGGGCGGAGGATATTGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A411Y3T7