Detailed information of TA system TA113303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3499291..3499881
• Replicon: chromosome
• Accession: NZ_CP033162
• Organism: Serratia sp. P2ACOL2

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: EBA31_RS17100
• Protein ID: WP_122079816.1
• Coordinates: 3499291..3499623 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: EBA31_RS17105
• Protein ID: WP_122079817.1
• Coordinates: 3499624..3499881 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBA31_RS17080	3495712..3496419	+	708	WP_020827675.1	heavy metal response regulator transcription factor	-
EBA31_RS17085	3496416..3497873	+	1458	WP_122079814.1	heavy metal sensor histidine kinase	-
EBA31_RS17090	3497875..3498798	-	924	WP_122079815.1	nitrogen assimilation transcriptional regulator NAC	-
EBA31_RS17100	3499291..3499623	-	333	WP_122079816.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EBA31_RS17105	3499624..3499881	-	258	WP_122079817.1	antitoxin	Antitoxin
EBA31_RS17115	3500078..3500281	-	204	WP_122079818.1	hypothetical protein	-
EBA31_RS17120	3500449..3501426	+	978	WP_122080650.1	DUF1852 domain-containing protein	-
EBA31_RS17125	3501454..3502485	+	1032	WP_122079819.1	methionine synthase	-
EBA31_RS17135	3503078..3503677	+	600	WP_046373148.1	hypothetical protein	-
EBA31_RS17140	3503711..3504643	-	933	WP_122079821.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11841.67 Da
• Isoelectric Point: 10.2965

>T113303 WP_122079816.1 NZ_CP033162:c3499623-3499291 [Serratia sp. P2ACOL2]
MDRGEIWLVSLDPTAGHEQSGSRPVLIVSKASFNKFTRLPVIVPVTSGGNFARMAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERLPDSIMNEVLGRLEAILT

Nucleotide

• Length: 333 bp

>T113303 NZ_CP033162:c3499623-3499291 [Serratia sp. P2ACOL2]
ATGGACAGAGGCGAAATCTGGCTCGTTTCACTTGATCCGACTGCCGGGCATGAGCAAAGCGGAAGCCGACCGGTCCTCAT
CGTATCGAAGGCATCGTTTAACAAGTTCACCCGGTTACCCGTTATTGTTCCTGTGACCAGCGGCGGAAACTTTGCCCGCA
TGGCGGGGTTTACCGTATCACTGGATGGCGCAGGGACGAAAACAACGGGTGTTATTCGTTGCGACCAGCCCAGAACCATT
GATATGGGGGCTCGTAGCGGCAAACGACTGGAACGTCTACCTGACAGTATCATGAACGAGGTGCTGGGCAGGCTGGAAGC
TATTCTGACCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9350.85 Da
• Isoelectric Point: 4.4488

>AT113303 WP_122079817.1 NZ_CP033162:c3499881-3499624 [Serratia sp. P2ACOL2]
MYTTRLKKVGGSVMLAVPPAVLKTLALSADSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHVEISEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT113303 NZ_CP033162:c3499881-3499624 [Serratia sp. P2ACOL2]
ATGTATACAACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCTGCGGTGCTGAAAACGCTGGCGCT
GTCGGCGGACAGTGAGGTTGGCATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGCTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGACCCGCATGTTGAAATAAGCGAGGAAGATCGGGAATGGATTGATGCGCCGGCTGTG
GGTAAGGAGATCTTGTAA