Detailed information of TA system TA113292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 918844..919471
• Replicon: chromosome
• Accession: NZ_CP033162
• Organism: Serratia sp. P2ACOL2

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: EBA31_RS04365
• Protein ID: WP_197821222.1
• Coordinates: 918844..919065 (-)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A379ZYL8
• Locus tag: EBA31_RS04370
• Protein ID: WP_020825515.1
• Coordinates: 919103..919471 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBA31_RS04350	914401..915744	-	1344	WP_122077512.1	NCS2 family permease	-
EBA31_RS04355	915909..917696	-	1788	WP_122077514.1	adenine deaminase	-
EBA31_RS04360	917835..918767	+	933	WP_122077516.1	LysR family transcriptional regulator	-
EBA31_RS04365	918844..919065	-	222	WP_197821222.1	hemolysin expression modulator Hha	Toxin
EBA31_RS04370	919103..919471	-	369	WP_020825515.1	Hha toxicity modulator TomB	Antitoxin
EBA31_RS04375	919634..919960	-	327	WP_122080582.1	hypothetical protein	-
EBA31_RS26880	920289..920435	+	147	WP_202914190.1	hypothetical protein	-
EBA31_RS04380	920432..921154	+	723	WP_048759568.1	ABC transporter ATP-binding protein	-
EBA31_RS04385	921141..921998	+	858	WP_041414279.1	metal ABC transporter permease	-
EBA31_RS04390	922024..922902	+	879	WP_020825520.1	metal ABC transporter substrate-binding protein	-
EBA31_RS04395	923015..923155	-	141	WP_012005563.1	type B 50S ribosomal protein L36	-
EBA31_RS04400	923168..923422	-	255	WP_020825521.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	910478..919987	9509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (14-70)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8727.21 Da
• Isoelectric Point: 7.9877

>T113292 WP_197821222.1 NZ_CP033162:c919065-918844 [Serratia sp. P2ACOL2]
ITPKVIMTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Nucleotide

• Length: 222 bp

>T113292 NZ_CP033162:c919065-918844 [Serratia sp. P2ACOL2]
ATAACACCAAAGGTTATCATGACTAAAACTGATTATCTGATGCGTTTACGTAAATGCACCACTATTGACACCCTTGAACG
CGTTATTGAAAAAAATAAGTATGAGCTTTCGGATGACGAATTGGAGCTGTTTTACTCTGCAGCTGACCACCGTCTGGCTG
AGCTCACCATGAATAAGCTCTACGATAAAATCCCAACGGCCGTTTGGAAGTTCGTACGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14189.89 Da
• Isoelectric Point: 4.5140

>AT113292 WP_020825515.1 NZ_CP033162:c919471-919103 [Serratia sp. P2ACOL2]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEGICTTMKS

Nucleotide

• Length: 369 bp

>AT113292 NZ_CP033162:c919471-919103 [Serratia sp. P2ACOL2]
ATGGATGAGTATTCGCCTAAAAGGCACGATATTGCTCAGCTGAAATTCTTGTGCGAAAATTTGTATGATGAAGGCATAGC
CACGCTGGGCGACAGCCACCACGGCTGGGTGAACGACCCAACGTCTTCCGTCAACCTCCAGCTTAATGAGTTGATTGAAC
ATATTGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGATGAAAGTGATTTATCGGAGTTAGTCGAGGAATATCTC
GATGATACCTACACGCTGTTCAGCAGTTACGGTATCAATGATTCCGATCTGCGTCGCTGGCAAAAAACCAAGGCGCGATT
ATTCAGAATGTTCTCAGGAGAGGGCATCTGTACGACAATGAAATCTTAG

Structures

Antitoxin

• AlphaFold DB: A0A379ZYL8