Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 12070..12713 Replicon plasmid p1ESCUMpO83_CORR
Accession NZ_CP033159
Organism Escherichia coli strain CM IVRI KOL-1

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag EAF06_RS23715 Protein ID WP_001034044.1
Coordinates 12297..12713 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag EAF06_RS23710 Protein ID WP_001261286.1
Coordinates 12070..12300 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAF06_RS23690 8701..9456 - 756 WP_000852146.1 replication initiation protein RepE -
EAF06_RS23695 10178..10984 - 807 WP_000016970.1 site-specific integrase -
EAF06_RS23700 10985..11290 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
EAF06_RS23705 11292..11510 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
EAF06_RS23710 12070..12300 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EAF06_RS23715 12297..12713 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
EAF06_RS23720 12788..14353 + 1566 WP_001128474.1 AAA family ATPase -
EAF06_RS23725 14338..15360 + 1023 WP_000361402.1 helicase UvrD -
EAF06_RS23730 15614..16300 - 687 Protein_19 IS1 family transposase -
EAF06_RS23735 16450..17147 - 698 Protein_20 IS1 family transposase -
EAF06_RS24980 17347..17460 + 114 Protein_21 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / blaTEM-1B / rmtB / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / tet(A) - 1..146286 146286


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T113278 WP_001034044.1 NZ_CP033159:12297-12713 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T113278 NZ_CP033159:12297-12713 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT113278 WP_001261286.1 NZ_CP033159:12070-12300 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT113278 NZ_CP033159:12070-12300 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References