Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 10985..11510 Replicon plasmid p1ESCUMpO83_CORR
Accession NZ_CP033159
Organism Escherichia coli strain CM IVRI KOL-1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag EAF06_RS23700 Protein ID WP_001159871.1
Coordinates 10985..11290 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag EAF06_RS23705 Protein ID WP_000813630.1
Coordinates 11292..11510 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAF06_RS23685 6947..8113 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
EAF06_RS23690 8701..9456 - 756 WP_000852146.1 replication initiation protein RepE -
EAF06_RS23695 10178..10984 - 807 WP_000016970.1 site-specific integrase -
EAF06_RS23700 10985..11290 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
EAF06_RS23705 11292..11510 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EAF06_RS23710 12070..12300 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
EAF06_RS23715 12297..12713 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
EAF06_RS23720 12788..14353 + 1566 WP_001128474.1 AAA family ATPase -
EAF06_RS23725 14338..15360 + 1023 WP_000361402.1 helicase UvrD -
EAF06_RS23730 15614..16300 - 687 Protein_19 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / blaTEM-1B / rmtB / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / tet(A) - 1..146286 146286


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T113277 WP_001159871.1 NZ_CP033159:c11290-10985 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T113277 NZ_CP033159:c11290-10985 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT113277 WP_000813630.1 NZ_CP033159:c11510-11292 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT113277 NZ_CP033159:c11510-11292 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References