Detailed information of TA system TA113274

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3668805..3669499
• Replicon: chromosome
• Accession: NZ_CP033158
• Organism: Escherichia coli strain CM IVRI KOL-1

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: EAF06_RS17505
• Protein ID: WP_001094398.1
• Coordinates: 3669131..3669499 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: EAF06_RS17500
• Protein ID: WP_077249034.1
• Coordinates: 3668805..3669110 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAF06_RS17465	3664755..3665210	+	456	WP_000581506.1	hypothetical protein	-
EAF06_RS17470	3665311..3665519	+	209	Protein_3393	DUF905 family protein	-
EAF06_RS17475	3665620..3666441	+	822	WP_001234565.1	DUF945 domain-containing protein	-
EAF06_RS17480	3666783..3667256	+	474	WP_000855059.1	antirestriction protein	-
EAF06_RS17485	3667272..3667748	+	477	WP_001424026.1	RadC family protein	-
EAF06_RS17490	3667817..3668038	+	222	WP_000692345.1	DUF987 domain-containing protein	-
EAF06_RS17495	3668057..3668701	+	645	WP_000086755.1	hypothetical protein	-
EAF06_RS17500	3668805..3669110	+	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EAF06_RS17505	3669131..3669499	+	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
EAF06_RS17510	3669820..3670158	-	339	Protein_3401	LysR family transcriptional regulator	-
EAF06_RS17515	3670209..3671165	-	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
EAF06_RS17520	3671175..3673373	-	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
EAF06_RS17525	3673370..3674326	-	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T113274 WP_001094398.1 NZ_CP033158:3669131-3669499 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T113274 NZ_CP033158:3669131-3669499 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT113274 WP_077249034.1 NZ_CP033158:3668805-3669110 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT113274 NZ_CP033158:3668805-3669110 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4