Detailed information of TA system TA113261

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 511403..512238
• Replicon: chromosome
• Accession: NZ_CP033158
• Organism: Escherichia coli strain CM IVRI KOL-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: EAF06_RS02540
• Protein ID: WP_000854761.1
• Coordinates: 511861..512238 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: EAF06_RS02535
• Protein ID: WP_001295723.1
• Coordinates: 511403..511771 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAF06_RS02510	508518..508676	+	159	WP_001323397.1	DUF905 family protein	-
EAF06_RS02515	508831..509649	+	819	WP_001234729.1	DUF945 domain-containing protein	-
EAF06_RS02520	509991..510464	+	474	WP_000855059.1	antirestriction protein	-
EAF06_RS02525	510480..510956	+	477	WP_001186774.1	RadC family protein	-
EAF06_RS02530	511019..511240	+	222	WP_000692345.1	DUF987 domain-containing protein	-
EAF06_RS02535	511403..511771	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EAF06_RS02540	511861..512238	+	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
EAF06_RS02545	512235..512437	+	203	Protein_498	hypothetical protein	-
EAF06_RS02550	512450..512563	+	114	WP_001161660.1	DUF957 domain-containing protein	-
EAF06_RS02555	512998..514533	-	1536	WP_001333339.1	IS66-like element ISEc22 family transposase	-
EAF06_RS02560	514582..514929	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
EAF06_RS02565	515051..515329	-	279	WP_001341328.1	transposase	-
EAF06_RS02570	515823..516212	+	390	WP_001445118.1	IS110 family transposase	-
EAF06_RS02580	516666..516848	+	183	WP_001016348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T113261 WP_000854761.1 NZ_CP033158:511861-512238 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T113261 NZ_CP033158:511861-512238 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT113261 WP_001295723.1 NZ_CP033158:511403-511771 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT113261 NZ_CP033158:511403-511771 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52