Detailed information of TA system TA113244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 26828..27396
• Replicon: plasmid pVPWZ1
• Accession: NZ_CP033143
• Organism: Vibrio parahaemolyticus strain 160807

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1Y1BA95
• Locus tag: D0871_RS26280
• Protein ID: WP_023624650.1
• Coordinates: 26828..27169 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: D0871_RS26285
• Protein ID: WP_122016154.1
• Coordinates: 27166..27396 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0871_RS26255	21928..22566	+	639	Protein_17	IS5 family transposase	-
D0871_RS26260	22900..23976	+	1077	WP_122016153.1	replication initiation protein	-
D0871_RS26270	25219..25950	+	732	WP_023624652.1	AHH domain-containing protein	-
D0871_RS26275	25950..26525	+	576	WP_017449240.1	hypothetical protein	-
D0871_RS26280	26828..27169	-	342	WP_023624650.1	endoribonuclease MazF	Toxin
D0871_RS26285	27166..27396	-	231	WP_122016154.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D0871_RS26290	27847..28572	+	726	WP_017449237.1	site-specific integrase	-
D0871_RS26295	28567..28950	-	384	Protein_24	recombinase family protein	-
D0871_RS26305	29553..29774	-	222	WP_023624649.1	hypothetical protein	-
D0871_RS26310	30084..30458	+	375	WP_127914005.1	hypothetical protein	-
D0871_RS26315	31019..32023	+	1005	WP_159408506.1	Ig-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106229	106229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-107)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12159.65 Da
• Isoelectric Point: 5.8124

>T113244 WP_023624650.1 NZ_CP033143:c27169-26828 [Vibrio parahaemolyticus]
MTYAVPEKGDVVVMSFNPQSGHEQAGDSRPGLVISPKEFNQATGFALVCPITNQSKGYPFEVEITGSQKTTGVVLADQVK
SLDWRARRARVVDSVSNETLGTVRDYVTAIANG

Nucleotide

• Length: 342 bp

>T113244 NZ_CP033143:c27169-26828 [Vibrio parahaemolyticus]
ATGACGTATGCAGTACCAGAGAAAGGCGATGTAGTAGTAATGAGTTTTAATCCGCAAAGCGGACACGAACAAGCGGGAGA
CAGTCGGCCTGGGCTGGTCATTTCCCCGAAAGAGTTTAACCAAGCAACTGGCTTTGCTCTGGTATGCCCCATAACCAACC
AGTCAAAAGGATATCCGTTTGAAGTTGAAATAACCGGAAGCCAAAAAACAACGGGTGTGGTGCTGGCTGACCAAGTGAAG
TCCTTGGATTGGCGCGCGCGTCGCGCTCGTGTGGTGGACTCCGTTTCAAACGAAACACTGGGGACTGTGCGGGATTATGT
CACAGCGATTGCTAATGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8499.87 Da
• Isoelectric Point: 6.4604

>AT113244 WP_122016154.1 NZ_CP033143:c27396-27166 [Vibrio parahaemolyticus]
MSQTIAKWGHSAAVRIPSKVLKNMDMAVGDKVEVKVFNRQIIIEPAKPTLAQILSQITDENRHDEVITETMGNELL

Nucleotide

• Length: 231 bp

>AT113244 NZ_CP033143:c27396-27166 [Vibrio parahaemolyticus]
ATGTCACAAACAATAGCTAAGTGGGGCCACAGCGCAGCTGTGCGTATACCTTCTAAGGTATTGAAAAATATGGATATGGC
GGTGGGTGACAAAGTGGAGGTTAAAGTGTTCAATCGACAAATTATCATTGAACCTGCAAAACCAACACTCGCCCAGATTT
TGAGTCAGATCACTGATGAAAACCGCCATGATGAAGTAATCACCGAAACTATGGGTAATGAATTGTTATGA

Structures

Toxin

• AlphaFold DB: A0A1Y1BA95