Detailed information of TA system TA113239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 89313..89881
• Replicon: plasmid pVOWZ1
• Accession: NZ_CP033139
• Organism: Vibrio owensii strain 1700302

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1Y1BA95
• Locus tag: D0812_RS29555
• Protein ID: WP_023624650.1
• Coordinates: 89540..89881 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: D0812_RS29550
• Protein ID: WP_122016154.1
• Coordinates: 89313..89543 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0812_RS29520	84686..85690	-	1005	WP_159408506.1	Ig-like domain-containing protein	-
D0812_RS29525	86251..86625	-	375	WP_127914005.1	hypothetical protein	-
D0812_RS29530	86935..87156	+	222	WP_023624649.1	hypothetical protein	-
D0812_RS29540	87759..88142	+	384	Protein_101	recombinase family protein	-
D0812_RS29545	88137..88862	-	726	WP_017449237.1	site-specific integrase	-
D0812_RS29550	89313..89543	+	231	WP_122016154.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D0812_RS29555	89540..89881	+	342	WP_023624650.1	endoribonuclease MazF	Toxin
D0812_RS29560	90184..90759	-	576	WP_017449240.1	hypothetical protein	-
D0812_RS29565	90759..91490	-	732	WP_023624652.1	AHH domain-containing protein	-
D0812_RS29575	92733..93809	-	1077	WP_122016153.1	replication initiation protein	-
D0812_RS29580	94143..94781	-	639	Protein_108	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..117926	117926

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-107)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12159.65 Da
• Isoelectric Point: 5.8124

>T113239 WP_023624650.1 NZ_CP033139:89540-89881 [Vibrio owensii]
MTYAVPEKGDVVVMSFNPQSGHEQAGDSRPGLVISPKEFNQATGFALVCPITNQSKGYPFEVEITGSQKTTGVVLADQVK
SLDWRARRARVVDSVSNETLGTVRDYVTAIANG

Nucleotide

• Length: 342 bp

>T113239 NZ_CP033139:89540-89881 [Vibrio owensii]
ATGACGTATGCAGTACCAGAGAAAGGCGATGTAGTAGTAATGAGTTTTAATCCGCAAAGCGGACACGAACAAGCGGGAGA
CAGTCGGCCTGGGCTGGTCATTTCCCCGAAAGAGTTTAACCAAGCAACTGGCTTTGCTCTGGTATGCCCCATAACCAACC
AGTCAAAAGGATATCCGTTTGAAGTTGAAATAACCGGAAGCCAAAAAACAACGGGTGTGGTGCTGGCTGACCAAGTGAAG
TCCTTGGATTGGCGCGCGCGTCGCGCTCGTGTGGTGGACTCCGTTTCAAACGAAACACTGGGGACTGTGCGGGATTATGT
CACAGCGATTGCTAATGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8499.87 Da
• Isoelectric Point: 6.4604

>AT113239 WP_122016154.1 NZ_CP033139:89313-89543 [Vibrio owensii]
MSQTIAKWGHSAAVRIPSKVLKNMDMAVGDKVEVKVFNRQIIIEPAKPTLAQILSQITDENRHDEVITETMGNELL

Nucleotide

• Length: 231 bp

>AT113239 NZ_CP033139:89313-89543 [Vibrio owensii]
ATGTCACAAACAATAGCTAAGTGGGGCCACAGCGCAGCTGTGCGTATACCTTCTAAGGTATTGAAAAATATGGATATGGC
GGTGGGTGACAAAGTGGAGGTTAAAGTGTTCAATCGACAAATTATCATTGAACCTGCAAAACCAACACTCGCCCAGATTT
TGAGTCAGATCACTGATGAAAACCGCCATGATGAAGTAATCACCGAAACTATGGGTAATGAATTGTTATGA

Structures

Toxin

• AlphaFold DB: A0A1Y1BA95