Detailed information of TA system TA113229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 944585..946146
• Replicon: chromosome
• Accession: NZ_CP033134
• Organism: Vibrio campbellii strain 170502

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A1W6M5G8
• Locus tag: D0784_RS04555
• Protein ID: WP_069504143.1
• Coordinates: 944818..946146 (+)
• Length: 443 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: D0784_RS04550
• Protein ID: WP_025787161.1
• Coordinates: 944585..944824 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0784_RS04530	939757..940854	-	1098	WP_122019020.1	alkene reductase	-
D0784_RS04535	940988..941869	-	882	WP_001259369.1	LysR family transcriptional regulator	-
D0784_RS04540	942058..943254	+	1197	WP_122019022.1	MFS transporter	-
D0784_RS04545	943338..944030	+	693	WP_122019024.1	SDR family oxidoreductase	-
D0784_RS04550	944585..944824	+	240	WP_025787161.1	helix-turn-helix domain-containing protein	Antitoxin
D0784_RS04555	944818..946146	+	1329	WP_069504143.1	type II toxin-antitoxin system HipA family toxin	Toxin
D0784_RS04560	946338..947660	-	1323	WP_062866033.1	site-specific integrase	-
D0784_RS04565	948054..949745	+	1692	WP_045417764.1	methyltransferase domain-containing protein	-
D0784_RS04570	949826..950254	+	429	WP_122020176.1	hypothetical protein	-
D0784_RS04575	950386..951108	+	723	WP_038891816.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	914918..950254	35336

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (151-399)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-55)

Sequences

Toxin

Protein

• Length: 443 a.a.
• Molecular weight: 49723.13 Da
• Isoelectric Point: 8.5824

>T113229 WP_069504143.1 NZ_CP033134:944818-946146 [Vibrio campbellii]
MVNRLHAYMNGVKVGVLEKRTNGAHVFTYDSGWVLAPNGRPISLSMPLRKDAYTGSEVINFFDNLLPDNQQIRDRIAARY
KAETKQPFDLLAKVGRDAVGAITLVPEGFDVDDHRTITAKALSEKELVRILKGYMEDAPLGMLKEEDDFRISIAGAQEKT
ALLFHDGRWMLPQGMTPTTHIIKLPIGEIVSQNNVIDLSASVDNELICMRLAKAFGIQTANCDIIRAGDVRALSVERFDR
KLSSDNSYYLRLPHEDFCQAKGIPSARKYEIDGGIGIEESIDILRYSYEINDTVTFARSQILFWLLAAIDGHGKNFSVSI
AASGRYQLAPLYDIISAHPLTGGRGLSERKIKMAMSLRGTSGRKWKWHQLQPRHFEDTFHTMNVPNIRREKVRLLLDEFE
SMAMEVIDSVGNSLPTDINESVRDKIFNGVLKTVKKIQLTKK

Nucleotide

• Length: 1329 bp

>T113229 NZ_CP033134:944818-946146 [Vibrio campbellii]
ATGGTGAATCGACTTCATGCTTACATGAATGGCGTAAAGGTTGGGGTACTTGAAAAGCGCACAAACGGCGCACATGTTTT
CACTTACGATTCGGGTTGGGTTTTAGCACCAAATGGTCGTCCTATCTCGCTTTCTATGCCTCTCAGGAAGGATGCGTATA
CTGGCAGTGAGGTTATTAACTTCTTTGATAACTTACTGCCAGATAATCAACAGATACGAGATAGAATAGCTGCTCGCTAT
AAAGCAGAGACTAAACAGCCATTCGACCTATTAGCTAAGGTTGGTCGAGATGCTGTAGGTGCTATCACGCTAGTTCCAGA
GGGCTTTGATGTAGATGATCATCGAACCATAACCGCAAAGGCGTTATCCGAAAAGGAATTGGTTAGAATCCTAAAGGGAT
ATATGGAGGACGCTCCTTTGGGGATGCTGAAAGAGGAAGATGATTTTAGGATCTCGATAGCGGGAGCGCAGGAAAAGACA
GCCTTACTGTTTCACGATGGAAGATGGATGCTACCACAGGGAATGACTCCAACTACGCATATTATCAAGCTGCCAATAGG
TGAAATTGTTTCTCAGAACAATGTTATTGATTTGTCTGCTAGTGTCGATAATGAGTTGATTTGTATGAGGCTAGCAAAGG
CGTTCGGCATTCAAACGGCAAACTGTGACATTATTCGTGCTGGTGATGTTCGTGCCTTGTCTGTTGAGCGATTTGACCGA
AAATTATCTTCTGATAACAGCTATTACTTGCGCTTGCCCCATGAGGATTTTTGCCAGGCAAAAGGGATTCCCTCAGCTAG
AAAATATGAAATTGACGGTGGTATCGGCATAGAAGAATCTATTGATATTTTGCGATACTCATACGAAATCAACGATACCG
TTACTTTTGCACGTTCTCAGATATTGTTTTGGCTACTAGCTGCCATTGACGGGCATGGAAAGAATTTCTCTGTAAGTATA
GCCGCATCTGGTCGATATCAGCTTGCACCACTGTATGACATTATCTCGGCACATCCACTGACAGGTGGGCGTGGTTTGAG
TGAGCGTAAGATCAAGATGGCAATGAGCTTACGTGGCACTAGTGGGCGAAAATGGAAGTGGCACCAACTTCAACCGCGCC
ATTTCGAAGATACCTTTCACACTATGAACGTCCCGAACATAAGGCGAGAAAAGGTTCGCTTATTGCTCGATGAGTTTGAA
TCGATGGCAATGGAGGTGATTGATTCTGTCGGAAACTCGCTACCAACTGATATCAATGAAAGCGTAAGAGATAAGATATT
TAACGGTGTCTTAAAGACCGTCAAGAAAATTCAATTGACAAAAAAATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8717.02 Da
• Isoelectric Point: 7.2039

>AT113229 WP_025787161.1 NZ_CP033134:944585-944824 [Vibrio campbellii]
MLVNTPTALGNALREARKESGLKQTDLGLRQATVSNFESNPEKSTIETLFKLLSINGLEMHIVPKGKHIIETKGAVDEW

Nucleotide

• Length: 240 bp

>AT113229 NZ_CP033134:944585-944824 [Vibrio campbellii]
ATGCTAGTCAACACACCGACAGCTCTCGGCAATGCGTTACGCGAAGCCCGAAAGGAAAGCGGCCTAAAGCAAACAGATCT
TGGTCTGCGACAAGCAACAGTATCTAATTTTGAAAGCAATCCTGAAAAATCCACGATAGAAACATTGTTCAAGCTCCTGT
CTATCAATGGACTAGAAATGCATATTGTGCCGAAAGGCAAGCACATTATTGAAACTAAAGGGGCTGTTGACGAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A1W6M5G8